Fig 1.
Species distribution of the gut microbiota of two endemic areas of strongyloidiasis in terms of the most abundant.
(A) Area graph showing relative proportions of the most abundant phyla (n = 5 in each group). (B) The top 10 families and (C) genera in terms of relative abundance in each sample group. (D) Hierarchical clustering heatmap of dominant species plotted by the absolute z-value. The colors in the heatmap refer to the species abundance, according to the color bar on the right. Numbers in the heatmap are reads per sample.
Fig 2.
Venn diagram showing numbers of unique, shared and core bacterial OTUs among sample groups.
Each ellipse represents one group. Values represent number of OTUs in each overlapping or unique segment. The lower panel graph represents total number of OTUs in each sample group.
Fig 3.
Measures of gut microbiome diversity and similarity between regions in samples from individuals infected and uninfected with Strongyloides.
(A) Boxplot of alpha diversity indices. The ACE and Chao1 indices estimate the OTU richness in samples. Shannon and Simpson indices reflect the OTU diversity in samples. The greater the ACE or Chao1 indices, the higher expected species richness of the microbiome; the smaller the Simpson or the greater the Shannon, the higher the diversity of the microbiome. Boxes represent the interquartile range, lines indicate medians, and whiskers indicate the range. Wilcoxon rank-sum test and Tukey’s test are used for statistically significant differences between groups (p < 0.05). (B) Boxplots based on weighted UniFrac distances to show the difference of beta diversity between groups (statistically significant differences between groups was set at p < 0.05). (C) Heatmap of the beta diversity matrix of the gut microbiota based on weighted UniFrac distances to reflect the dissimilarity between samples groups.