Fig 1.
Targeted patch-clamp recordings in acute midbrain slices.
A. Schematics illustrating the use of acute midbrain slices in combination with other techniques to target neurons in a midbrain area of interest (PAG, in yellow). B. Example images from experiments targeting midbrain neurons in different anatomical subdivisions of the PAG in the same slice. Scale bars are 1 mm. Abbreviations indicate the different PAG subdivisions: dorsomedial (dmPAG), dorsolateral (dlPAG), lateral (lPAG), and ventrolateral (vlPAG). C. (Left) Spontaneous electrical activity of a PAG neuron during a loose-seal cell-attached recording. (Right) Voltage response to step current injections of a PAG neuron during a whole-cell recording.