Fig 1.
Map of sampling localities for Bd in southern Nevada (A) and northwestern Pennsylvania (B). Individual sampling localities are numbered and represented with a pie chart that shows the proportion of samples belonging to each Bd-GPL sub-lineage as determined using the DAPC clustering analysis. Unassigned samples (in grey) are those with a posterior probability of assignment to either group < 0.99. Pie charts are scaled in size according to the number of samples collected at that site. (C) PCA calculated from 129 SNPS using the combined dataset of southern Nevada (N = 52, squares), northwestern Pennsylvania (N = 71, circles), and global reference samples (N = 26, triangles). Symbols and ellipses colored as in maps above. Satellite imagery courtesy of the Earth Science and Remote Sensing Unit, NASA Johnson Space Center (http://eol.jsc.nasa.gov).
Fig 2.
(A) Phylogenetic tree and (B) PCA for 52 Bd samples collected in southern Nevada.
Colors represent the locality where the Bd was sampled (numbered as in Fig 1) and symbols represent host species. Tree is calculated using Astral-III and includes and additional 13 reference samples representing all known clades of Bd. Nodes are labeled with posterior probability and those with posterior < 0.7 have been collapsed.
Fig 3.
(A) Phylogenetic tree and (B) PCA for 71 Bd samples collected in northwestern Pennsylvania.
Colors represent the locality where the Bd was sampled (numbered as in Fig 1) and symbols represent host species. Tree is calculated using Astral-III and includes and additional 13 reference samples representing all known clades of Bd. Nodes are labeled with posterior probability and those with posterior < 0.7 have been collapsed.
Fig 4.
Alleles for PA Site 1 (left top), PA Site 3 (left middle), PA Site 4 (left bottom), NV Site 1 (right top), and NV Site 4 (right bottom).
Each row represents an individual sample, and each numbered column represents a nuclear locus that has an informative GPL1/GPL2 allele (N = 24). Loci are organized according to position along the Bd genome, with the yellow dashed indicating a change to a new chromosome and the chromosome number labeled along the top. Samples are grouped by host species (“species” column) and labeled with the DAPC assigned lineage (“lineage” column). A red box indicates a Bd-GPL2 allele for that sample at that locus, a blue box represented a Bd-GPL1 allele, a gray box indicates both alleles are present, and a white box indicates missing data. Representative sequences for each allele are included in the supplemental materials.