Fig 1.
Experimental outline of the study.
Lungs from donor rats were randomly assigned to one of four experimental groups (n = 7/group): 1) healthy, directly procured lungs, 2) lungs procured from rats subjected to 3 hours of brain death (BD) and 1 hour cold storage (CS), 3) healthy, directly procured lungs subjected to 6 hours ex vivo lung perfusion (EVLP) and 4), lungs procured from rats subjected to 3 hours of BD, 1 hour CS and 6 hours EVLP.
Fig 2.
Schematic overview of the rat ex vivo lung perfusion model.
Lungs from healthy or brain-dead donor rats were subjected to 6 hours ex vivo lung perfusion (EVLP). The EVLP circuit consisted of a (1) graft humidity chamber, (2) reservoir, (3) roller pump, (4) leukocyte filter, (5) deoxygenator, (6) heat exchanger, (7) funnel, (8) pressure sensor, (9) ventilator, and a (10) water bath.
Table 1.
List of reagents for ex vivo lung perfusion.
Table 2.
List of instruments for the ex vivo lung perfusion model.
Fig 3.
Experimental groups with number of rats utilized and rationale for exclusion.
Lungs from donor rats were randomly assigned to one of four experimental groups (n = 7/group): 1) healthy, directly procured lungs, 2) lungs procured from rats subjected to 3 hours of brain death (BD) and 1 hour cold storage (CS), 3) healthy, directly procured lungs subjected to 6 hours ex vivo lung perfusion (EVLP) and 4), lungs procured from rats subjected to 3 hours of BD, 1 hour CS and 6 hours EVLP. In total, 38 rats were utilized for the establishment of a stable BD and EVLP protocol, of which 10 rats were excluded. Eventually, 7 rats per group were included in the final protocol.
Fig 4.
Ventilation parameters and inflammatory status after lung procurement.
Lungs were procured from either healthy rats (experimental group 1) or rats subjected to 3 hours of brain death (BD, experimental group 2). (A) Pulmonary Inspiratory Pressure (PIP) required to maintain tidal volume at 7 ml/kg of bodyweight and dynamic compliance (Cdyn) of healthy donor lungs 1) versus donor lungs subjected to 3 hours of BD, at time of lung procurement. (B) Pro-inflammatory gene expressions of TNF-α, IL-1β, IL-6, MCP-1 and C3 of healthy donor lungs versus donor lungs subjected to 3 hours of BD. ** p<0.01 in healthy donor lungs versus lungs from brain-dead donors.
Fig 5.
Lung morphology after lung procurement.
Lungs were procured from either healthy rats (experimental group 1) or rats subjected to 3 hours of brain death (BD, experimental group 2). Histological lung injury was scored after staining for hematoxylin and eosin (H&E). (A) Quantification of lung injury scores in H&E-stained lung slides. (B-C) Representative H&E-stained slices of healthy donor lungs and lungs from brain-dead donors. ** p<0.01 in healthy donor lungs versus lungs from brain-dead donors.
Fig 6.
Ventilation and perfusion performance during ex vivo lung perfusion.
Lungs from healthy donor rats or rats subjected to 3 hours of brain death (BD) and 1 hour cold storage (CS) were ex vivo perfused for 6 hours (EVLP, experimental group 3 and 4). (A) Positive Inspiratory Pressure (PIP) required to maintain tidal volume at 7 ml/kg of bodyweight over time, during EVLP. (B) Dynamic compliance (Cdyn) of donor lungs over time, during EVLP. (C) Oxygenation capacity of donor lungs as reflected by PaO2/FiO2 ratio. (D) Perfusion flow of donor lungs over time, during EVLP. * p<0.05 in healthy donor lungs versus lungs from brain-dead donors subjected to EVLP. ** p<0.01 in healthy donor lungs versus lungs from brain-dead donors subjected to EVLP.
Fig 7.
Metabolic profile and inflammatory status after ex vivo lung perfusion.
Lungs from healthy donor rats or rats subjected to 3 hours of brain death (BD) and 1 hour cold storage (CS) were ex vivo perfused for 6 hours (EVLP, experimental group 3 and 4). (A) Cumulative glucose consumption of healthy donor lungs versus lungs from brain-dead rats, during EVLP. (B) Cumulative lactate production of healthy donor lungs versus lungs from brain-dead rats, during EVLP. (C) Pro-inflammatory gene expressions of TNF-α, IL-1β, IL-6, MCP-1 and C3 in donor lungs, after 6 hours of EVLP. * p<0.05 in healthy donor lungs versus lungs from brain-dead donors subjected to EVLP. ** p<0.01 in healthy donor lungs versus lungs from brain-dead donors subjected to EVLP.
Fig 8.
Lung morphology after ex vivo lung perfusion.
Lungs from healthy donor rats or rats subjected to 3 hours of brain death (BD) and 1 hour cold storage (CS) were ex vivo perfused for 6 hours (EVLP, experimental group 3 and 4). Histological lung injury was scored after staining for hematoxylin and eosin (H&E). (A) Quantification of lung injury scores after EVLP in H&E-stained lung slides. (B-C) Representative H&E-stained slices of healthy donor lungs and lungs from brain-dead donors, after EVLP. ** p<0.01 in healthy donor lungs versus lungs from brain-dead donors subjected to EVLP.
Table 3.
Donor type characteristics of reported rat ex vivo lung perfusion models.