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Table 1.

Composition of the four perfusion solutions.

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Fig 1.

Schematic figure of the normothermic perfusion circuit.

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Table 2.

Baseline information.

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Table 2 Expand

Fig 2.

Renal arterial flow (ml/min/100g) of the four experimental groups during NMP (mean + SD).

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Fig 2 Expand

Table 3.

Functional and injury parameters throughout perfusion.

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Table 3 Expand

Fig 3.

Cumulative diuresis (ml) (A) and AUC (B) of the four experimental groups during NMP (mean + SD).

** indicates a p-value of ≤ 0.01. *** indicates a p-value of ≤ 0.001.

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Fig 3 Expand

Fig 4.

Sodium and potassium levels (mmol/L) of the four experimental groups during NMP (mean + SD).

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Fig 5.

pH of the four experimental groups during NMP (mean + SD).

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Fig 6.

Mean creatinine clearance (ml/min) (A), AUC of creatinine clearance (B), mean fractional excretion of creatinine per 100g (FE creatinine/100g) (C), AUC of FE creatinine (D), mean fractional excretion of sodium (FENa+) (E), and AUC of FENa+ (F) of the four experimental groups during NMP (+ SD).

* indicates a level of significance of p ≤ 0.05.

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Fig 6 Expand

Fig 7.

ASAT levels (U/l) (A), AUC of ASAT (B), LDH levels (U/l) (C), and AUC of LDH (D) of the four experimental groups during NMP (mean + SD).

* indicates a level of significance of p ≤ 0.05.

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Fig 7 Expand

Fig 8.

Mean NAG levels (U/L) (A) and AUC of NAG (B), mean MDA levels (uM) (C), and AUC of MDA (D) of the four experimental groups during NMP (+ SD).

* indicates a level of significance of p ≤ 0.05. ** indicates a p-value of ≤ 0.01. *** indicates a p-value of p ≤ 0.001.

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Fig 8 Expand

Fig 9.

Scores on necrosis, tubular and glomerular dilatation at t = 0 and t = 420 (mean + SD).

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Fig 10.

Bax (A) and Bcl-2 (B) levels at t = 0 and t = 420 (mean + SD). Bax/Bcl-2 ratios (C) are displayed per group.

* indicates a level of significance of p ≤ 0.05. ** indicates a p value of ≤ 0.01. *** indicates a p value of p ≤ 0.001.

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Fig 10 Expand