Fig 1.
Representative phenotype of Phalaenopsis plant types Grandiflora (A) and Multiflora (B).
Table 1.
Overview of experimental set-up and growth conditions, highlighting differences between setup of vegetative experiment I and II.
Fig 2.
Trait component analysis of vegetative Phalaenopsis plants.
Main effects of light (A, B) and temperature (C, D). Plants were grown in climate chambers under LED lighting for 14 hours per day at a PPFD of 60 or 140 μmol m-2 s-1. In experiment I (A,C; n = 3; 5–7 plants per statistical replicate per genotype), plants of 19 genotypes were grown for 19 weeks at either 27°C or 31°C. In experiment II (B,D; n = 5; per genotype) 14 genotypes were grown for 15 weeks at either 26°C or 30°C. Data is averaged over genotypes, percentages represent average change per trait to reference light intensity (60 μmol m-2 s-1; A, B) or temperature (27°C, C;26° C, D). NS, *,**,*** are not significant or significant at p<0.05, p<0.01 and p<0.001, respectively. For temperature, significance was determined at α<0.01, for light at α<0.05.
Fig 3.
Trait component analysis of flowering Phalaenopsis plants.
Effects of light (A) and temperature (B) treatments applied during the vegetative phase. During the vegetative phase, plants were grown in climate chambers for 23 weeks at either 26°C or 30°C, and a PPFD of 60 or 140 μmol m-2 s-1 for 14 hours per day. Plants from all treatments were then moved to the greenhouse for flower induction (8 weeks) and subsequent flowering phase. Plants of a genotype were harvested, when 2/3 reached the consumer-ready stage, defined as plants having two open flowers per plant (15–21 weeks, depending on genotype). Percentages represent average change per trait to either vegetative reference temperature (26°C) or light intensity (60 μmol m-2 s-1). NS, *,**,*** are not significant or significant at p<0.05, p<0.01 and p<0.001, respectively (n = 10 per genotype). For temperature, significance was determined at α<0.01, for light at α<0.05.
Fig 4.
Genotypic variation in vegetative Phalaenopsis plants in response to light.
Plants were grown in climate chambers under LED lighting for 14 hours per day at a PPFD of 60 or 140 μmol m-2 s-1. In experiment I (A; n = 3; 5–7 plants per statistical replicate per genotype), plants of 19 genotypes were grown for 19 weeks at either 27°C or 31°C. In experiment II (B; n = 5; per genotype) 14 genotypes were grown for 15 weeks at either 26°C or 30°C. Data is averaged over temperature, and represents relative change per trait to light intensity (60 μmol m-2 s-1). Similar colours are similar genotypes, also in Figs 5 and 6.
Fig 5.
Genotypic variation in vegetative Phalaenopsis plants in response to temperature.
Plants were grown in climate chambers under LED lighting for 14 hours per day at a PPFD of 60 or 140 μmol m-2 s-1. In experiment I (A; n = 3; 5–7 plants per statistical replicate per genotype), plants of 19 genotypes were grown for 19 weeks at either 27°C or 31°C. In experiment II (B; n = 5; per genotype) 14 genotypes were grown for 15 weeks at either 26°C or 30°C. Data is averaged over temperature, and represents relative change per trait to light intensity (60 μmol m-2 s-1). Similar colours are similar genotypes, also in Figs 4 and 6.
Table 2.
Significance of genotypic variation to temperature and light.
Effects on vegetative growth traits of 19 (experiment I) or 14 (experiment II) different genotypes of Phalaenopsis plants.
Table 3.
Effect of plant type and genotypic variation and its interaction with temperature and light in flowering Phalaenopsis.
Treatments applied in vegetative phase on after-effects during flowering of 14 different genotypes of Phalaenopsis plants.
Fig 6.
Genotypic variation in flowering Phalaenopsis plants of experiment II.
During the vegetative phase, plants were grown in climate chambers for 15 weeks at either 26°C or 30°C and a PPFD of 60 or 140 μmol m-2 s-1 for 14 hours per day. Plants from all treatments were simultaneously moved to the greenhouse for cooling and flowering phase until auction-ready; see material and methods for details. Data is averaged either over temperature, and represents relative change per trait to light intensity light intensity (60 μmol m-2 s-1)(A) or is averaged over light, and represents relative change per trait to temperature (26°C)(B)(n = 10), as applied during vegetative growth. Similar colours are similar genotypes, also in Figs 4 and 5.
Fig 7.
Trait correlation matrix of vegetative Phalaenopsis plants.
Plants were grown in climate chambers under LED lighting for 14 hours per day at a PPFD of 60 or 140 μmol m-2 s-1. In experiment I (A; n = 3; 5–7 plants per statistical replicate per genotype), plants of 19 genotypes were grown for 19 weeks at either 27°C or 31°C. In experiment II (B; n = 5; per genotype) 14 genotypes were grown for 15 weeks at either 26°C or 30°C. Data is pooled over genotypes and growth treatment. Colours represent either negative (red) or positive (blue). Significant correlations are marked *,**,*** at p<0.05, p<0.01 and p<0.001, respectively. RGR = relative growth rate, LMA = leaf mass area, dw = dry weight.
Fig 8.
Trait correlation matrix of flowering Phalaenopsis plants in experiment II.
During the vegetative phase, plants were grown in climate chambers for 23 weeks at either 26°C or 30°C and a PPFD of 60 or 140 μmol m-2 s-1 for 14 hours per day. Then, plants from all treatments were simultaneously moved to the greenhouse for flower induction and flowering phase until consumer-ready. Data is pooled over genotypes and growth treatment. Colours represent either negative (red) or positive (blue) correlations. Significant correlations are marked *,**,*** at p<0.05, p<0.01 and p<0.001, respectively. RGR = relative growth rate.
Fig 9.
Correlation matrix of vegetative (V) traits with flowering Phalaenopsis plants in experiment II.
Data is averaged over genotypes and growth treatment. During the vegetative phase, plants were grown in climate chambers for 15 weeks at either 26°C or 30°C and a PPFD of 60 or 140 μmol m-2 s-1 for 14 hours per day, after which randomly selected plants were harvested. Plants were then allowed to grow for another 8 weeks, before plants from all treatments were simultaneously moved to the greenhouse for flower induction and flowering phase until consumer-ready. Colours represent either negative (red) or positive (blue) correlations. Significant correlations are marked *,**,*** at p<0.05, p<0.01 and p<0.001, respectively. RGR = relative growth rate, LMA = leaf mass area, dw = dry weight.