Fig 1.
Sample collection flow diagram.
Flow diagram of biological sample collection, and study sample inclusion.
Table 1.
Variable summary of potential predictors of RNA quality.
Table 2.
Summary of RNA quality outcomes.
Fig 2.
Paired t-test comparing mean RNA purity (260/280 optical absorbency ratios) in the 2011 and 2018 RNA isolations.
Fig 3.
Paired t-test comparing mean RNA yield in the 2011 and 2018 RNA isolations.
Fig 4.
RNA purity linear regression results.
Association between the difference in RNA purity (2011 260/280 ratio minus 2018 260/280 ratio) and length of storage time. The negative slope implies that the difference between 2011 and 2018 increased with storage time, with the 2018 purity increasing over time.
Fig 5.
RNA yield linear regression results.
Association between the difference in RNA quantity (2011 μg /tube minus 2018 μg /tube) and length of storage time. The negative slope implies that the difference between 2011 and 2018 increased with storage time, with the 2018 quantity increasing over time.
Fig 6.
RNA integrity linear regression results.
Association between the RNA integrity number and length of storage time.