Table 1.
Identification of the isolates by MALDI TOF-MS or 16S rRNA gene sequencing.
Table 2.
Sequencing, assembly and annotation results for MTZ052 and MTZ096 genomes.
Fig 1.
Phylogenetic analysis of MTZ052 and MTZ096.
Maximum likelihood-based inference phylogenetic trees were reconstructed based on 57 gene families shared by the indicated bacterial species. Millisia brevis NBRC 105863 and Skermania piniformis NBRC 15059 were used as an outgroup. Branch bootstrap values are at least 80%, unless otherwise indicated.
Fig 2.
Schematic comparison of the two main gene clusters for alkane degradation in MTZ052 and MTZ096.
(A) Alkane hydroxylase cluster genes: alkane monooxygenase (red), rubredoxin (blue) and reductase (yellow). (B) CYP153 cluster genes: 2Fe-2S ferredoxin (red), cytochrome P450 (blue) and ferredoxin reductase (yellow). Transcriptional regulators colored in gray and pink are indicated. Comparison between the strains studied in this work (underlined) and available strains in IMG/M are shown.
Table 3.
Similarities among amino acid sequences encoded by the AH system in MTZ096 and NBRC 108236 (G. sihwensis).
Table 4.
Similarities among amino acid sequences encoded by the CYP153 gene clusters in MTZ052, MTZ096 and NBRC 108238 (G. paraffinivorans).
Table 5.
Biodegradation indexes of n-hexadecane of MTZ052 and MTZ096 strains during 28 days of chromatographic monitoring.
Fig 3.
Transcriptional pattern by real-time PCR.
AH, (B) CYP153 gene clusters in G. sihwensis MTZ096 and (C) transcriptional pattern of CYP153 gene cluster in G. paraffinivorans MTZ052.