Table 1.
Dates of flowering periods of the mapping population GF.GA-47-42 x ‘Villard Blanc’ and the amount of global radiation at the location of the vineyards (Geilweilerhof) if available.
Table 2.
Samples collected from grapevine genotype GF.GA-47-42 for the analysis of trends in gene expression levels.
Fig 1.
Workflow using the Genome Analysis Toolkit (GATK).
The workflow uses the high-coverage genotype sequence variation information and the family relationship for phasing.
Fig 2.
Correlation between alleles of FTC target genes and flowering time phenotype.
Given are the allele constitutions of the parental lines for each gene and the allele counts of the amplicon sequenced F1 individuals. The median of flowering time (calculated from days after January 1st of the years 1999 and 2010–2016) of individuals carrying the counted is given in brackets. The higher the value of the median, the later the flowering phenotype of the F1 individuals. Color coded are the p-values for the E alleles and L alleles in the up to 35 F1 individuals. Significant correlation values are in bold and italic. Genes located in QTL regions are marked in grey. Differences in allele counts between the years are due to missing data points. “E” alleles are inherited from GF.GA-47-42, while "L" alleles originate from ‘Villard Blanc’. "N" means that both GF.GA-47-42 and ‘Villard Blanc’ share one or more alleles. "E0": E1 = E2, "L0": L1 = L2, "N1": E1 = L1, "N2": E2 = L2. "N": L2 = E1 or E2 = L1, "Na": E1 = E2 = L1, "Nb": E1 = E2 = L2. „n.d.”: not determined. Further explanations are given in S4 Table.
Table 3.
P-values of the correlation between the E1 allele distribution of VvWNK6 and VvTM6 in relation to different sets of phenotypic data using 35 amplicon sequenced F1 individuals.
Fig 3.
Distribution of allele combinations for VvWNK6 (chr 4) among 35 selected individuals of the mapping population GF.GA-47-42 x ‘Villard Blanc’.
The date of flowering was counted in days from the 1st of January and the data was subsequently classified according to six stages for flowering time following (1 = very early flowering; 2 = early flowering; 3 = medium early flowering; 4 = medium late flowering; 5 = late flowering; 6 = very late flowering). For visualization flowering classes 1 and 2, 3 and 4, and 5 and 6 were merged.
Table 4.
Comparison of the expected and observed allele sizes (bp) and segregation patterns of several FTC target genes.
Table 5.
P-values of correlation between alleles and the phenotype of flowering time.
Fig 4.
Heatmap of FTC candidate genes showing variations in their expression over consecutive time points of bud development from dormancy until appearance of inflorescence in grapevine variety GF.GA-47-42.
Time series from December 20th, 2012 to May 3rd, 2013. LFC-threshold: 2 = expression fourfolded, -2 = expression quartered. Shown are rlog transformed counts.
Fig 5.
Heatmap of gene expression of amplicon sequenced FTC candidate genes in GF.GA-47–42 at different developmental stages of buds and inflorescences.
LFC-threshold: 1 = expression doubled, -1 = expression halved. rlog transformed counts are shown.
Fig 6.
Heatmap of FTC candidate genes showing expression variations between leaves and their prompt buds/ inflorescences.
LFC-threshold: 2 = expression fourfolded, -2 = expression quartered. Shown are rlog transformed counts.