Fig 1.
Scopolamine caused increases in alpha, theta, and delta power bands of the qEEG spectra, in a dose-dependent manner.
Scopolamine (25–100 μg/kg) was subcutaneously administered to cynomolgus monkeys. After treatment with scopolamine or vehicle, (A) alpha, (B) theta, and (C) delta power bands of qEEG spectra were measured from 30 to 240 min. Results represent mean ± SEM of AUC between 30 and 210 min after treatment for 4 monkeys in each group. *P ≤ 0.05 versus vehicle-treated group by paired t-test. AUC, area under the curve; qEEG, quantitative electroencephalogram; SEM, standard error of the mean.
Fig 2.
Donepezil at 3 mg/kg inhibited the scopolamine-induced qEEG spectral changes.
Scopolamine (25 μg/kg, s.c.) and donepezil (3 mg/kg, p.o.) were simultaneously administered to cynomolgus monkeys. After treatment with drugs or vehicle, (A) alpha, (B) theta, and (C) delta power bands of qEEG spectra were measured from 30 to 240 min. Results represent mean ± SEM of AUC between 30 and 210 min after treatment for 4 monkeys in each group. *P ≤ 0.05 versus vehicle-treated group by paired t-test. #P ≤ 0.05 versus scopolamine-treated group by paired t-test. AUC, area under the curve; p.o., oral administration; qEEG, quantitative electroencephalogram; SEM, standard error of the mean; s.c., subcutaneous administration.
Fig 3.
Xanomeline at 1 mg/kg tended to attenuate the scopolamine-induced qEEG changes.
Scopolamine (25 μg/kg, s.c.) and xanomeline (1 mg/kg, s.c.) were simultaneously administered to cynomolgus monkeys. After treatment with drugs or vehicle, (A) alpha, (B) theta, and (C) delta power bands of qEEG spectra were measured from 30 to 240 min. Results represent mean ± SEM of AUC between 30 and 210 min after treatment for 4 monkeys in each group. AUC, area under the curve; qEEG, quantitative electroencephalogram; SEM, standard error of the mean; s.c., subcutaneous administration.
Fig 4.
TAK-071 at 0.3–3 mg/kg suppressed the scopolamine-induced qEEG changes.
Scopolamine (25 μg/kg, s.c.) and TAK-071 (0.3–3 mg/kg, p.o.) were simultaneously administered to cynomolgus monkeys. After treatment with drugs or vehicle, (A) alpha, (B) theta, and (C) delta power bands of qEEG spectra were measured from 30 to 240 min. Results represent mean ± SEM of AUC between 30 and 210 min after treatment for 3–4 monkeys in each group. *P ≤ 0.05 versus vehicle-treated group by paired t-test. #P ≤ 0.05 versus scopolamine-treated group by paired t-test. AUC, area under the curve; p.o., oral administration; qEEG, quantitative electroencephalogram; SEM, standard error of the mean; s.c., subcutaneous administration.