Fig 1.
(a) The complete IUCN distribution of O. roylei is shown in grey [32]. (b) The three sampling locations are indicated. Reprinted from ArcGIS under a CC BY license, with permission from Esri, original copyright 2009.
Table 1.
Pika sample localities.
Fig 2.
Volcano plot of differential expression between the 5,000 m site and the two lower-elevation sites.
Each point is a transcript. The x-axis is the beta value which is the natural log of the fold difference and the y-axis is the–log10(qval). The q-value is the multiple test corrected p-value. Colored points are significantly differentially expressed between the high-elevation group and the other locations at a q-value of < 0.05. Transcripts falling above zero on the x-axis (green) are upregulated in the 5,000 m samples and transcripts falling below zero (blue) are down-regulated. Transcripts driving the enrichment of the OXPHOS pathway are shown in dark green circles. Transcripts identified in the text to be of particular interest based on our literature search are also shown with a dark green or dark blue point, with different shapes indicating what each gene is related to.
Fig 3.
Heat map of transcripts that were significantly differentially expressed in the 5,000 m samples.
Each row is an individual, with individuals from the 5,000 m site indicated in blue, the 4,000 m site indicated in teal, and 3,600 m site indicated in black. Each column is a transcript and the mean number of transcripts per million (TPM), a measure of expression level, for each transcript is indicated. In the heat map itself, lower expression is indicated in blue and higher expression is indicated in green.
Table 2.
Genes upregulated in the 5,000 m samples.
Table 3.
GO terms and KEGG pathways significantly enriched in genes upregulated in the 5,000 m individuals.
Table 4.
Genes down-regulated in the 5,000 m samples.