Fig 1.
Microglanis cottoides, MZUEL 7453, 54.6 mm SL.
Specimen collected in the Arroio Divisa, Camaquã River basin, Cristal, RS, 30o54'5.6"S 52o05'18.9"W (Photo by O.A. Shibatta).
Fig 2.
Map with collection points of Microglanis in the southeastern and southern states of Brazil.
The acronyms SP, PR, SC and RS, refer to the states of São Paulo, Paraná, Santa Catarina and Rio Grande do Sul, respectively. The red circles correspond to the samples from Camaquã river basin, drainage of the type locality of M. cottoides.
Table 1.
Mean genetic distances using the K2P model (Kimura-2-parameters) among populations of Microglanis cottoides and other species of Microglanis obtained with COI data of 119 individuals.
Fig 3.
Median-joining networks and Bayesian phylogenetic tree of Micriglanis obtained with COI data.
(A) Median-Joining networks among haplotypes. Each circle represents a unique haplotype with circle sizes being proportional to their frequencies. Each color represents a species. The numbers between haplotypes correspond to mutational steps. Haplotypes: H1 = Ribeira; H2 = Guaratuba + Paranaguá + Itapocu; H3 = Paranaguá; H4 = Paranaguá; H5 = Madre; H6 = Araranguá; H7 = Araranguá; H8 = M. cibelae (Mampituba); H9 = M. cibelae (Tramandaí); H10 = Patos (Camaquã); H11 = Negro + Uruguay; H12 = Uruguay; H13 = Uruguay; H14 = Microglanis sp. Uruguay; H15 = M. parahybae: H16 = M. malabarbai; H17 = M. malabarbai; H18 = M. garavelloi. (B) Bayesian tree. Node bars represent the threshold time for each cladogenetic event. The values above the branches indicate the posterior probability (pp). * Individuals collected in their respective type locality.
Table 2.
Morfometry of Microglanis cottoides (n = 19), M. cibelae (n = 15) and M. malabarbai (n = 13).
Fig 4.
Dispersion of individual scores on the second and third Principal Components axis.
Microglanis cottoides (red, n = 19), M. cibelae (pink, n = 15) and M. malabarbai (blue, n = 13).
Table 3.
Variables loadings on first three principal components axis of cobined samples of Microglanis cottoides, M. cibelae, and M. malabarbai.
Fig 5.
Box plot of the lateral line pore counts.
Microglanis cottoides (n = 17), M. cibelae (15), and M. malabarbai (n = 12). Point represents outlier.