Fig 1.
Karyotype and FISH mapping probed with PCR amplicons of LG1-linked scaffolds in Japanese eel.
Giemsa-stained karyotype of a wild-captured Japanese eel (A). Hybridization of rhodamine-labeled scaffold_127 amplicons (red signals) on a DAPI-stained metaphase spread (B). Hybridization of Alexa Fluor® 488-labeled scaffold_10233 (green signals) and rhodamine-labeled 12248 (red signals) amplicons on a DAPI-stained metaphase spread (C), and image of fluorescent signals only on the same metaphase spread (D). Arrowheads indicate hybridization signals. Scale bars represent 10 μm.
Fig 2.
Chromosome sorting in Japanese eel.
Bivalent profile of flow sorting with forward scatter (FSC) and side scatter (SSC) is shown (A). Bivalent profile of flow sorting with chromomycin A3 (CMA3) and Hoechst33258 (Hoechst) for samples that pass region 2 (R2) is shown (B). Droplets that pass region 1 (R1) and R2 of (A) contained debris of nuclei and dividing cells (C), and chromosomes (D), respectively.
Table 1.
Result of read mapping to the reference genome sequences of Japanese eel.
Fig 3.
Chromosome painting with WGA products from single chromosomes.
Chromosome painting probed with WGA products from sorted single chromosome samples EE2-sc-5sc (A), 6 (B), 8 (C), and 15 (D) on metaphase spreads from a Japanese eel cell line (EE2). The probes were labeled with rhodamine (red signals; A-C) or Alexa Fluor® 488 (green signals; D). Chromosome painting probed with a mixture of the four products on a metaphase spread of a wild-captured animal (E). Scale bars represent 10 μm.
Fig 4.
FISH mapping with putative LG1-linked scaffolds in Japanese eel.
PCR amplicons from scaffold_12 (A), 135 and 297 (B), and 256 and 997 (C) are mapped to chromosome 5. The amplicons of scaffold_12 (A), 135 (B), and 256 (C) were labeled with rhodamine (red signals), and scaffold_297 (B) and 997 (C) were labeled with Alexa Fluor® 488 (green signals). Arrowheads indicate hybridization signals. Scale bars represent 10 μm.