Table 1.
Occurrence of lmo2550 gene mutation in L. monocytogenes serotype 1/2a.
Accession numbers of the nucleotide sequence data refer to S1 Table.
Fig 1.
Adhesion to the formation of the 48-h biofilms by the DSS 1130 BFA2, EGD-eΔlmo2549 and EGD-eΔlmo2550 mutants and the wild-type EGD-e strain grown on stainless steel at 30°C in MCDB202 medium.
(A) Enumeration of viable culturable population at t = 3 h; 6 h, 24 h, 30 h and 48 h. The error bars represent the standard deviation (n = 3) and * represent significant difference. (B) Observations by epifluorescence microscopy after staining at acridine orange at t = 3 h, 6 h, 24 h, 30 h and 48 h. The size bar of 100 Pixel indicated 5 μm.
Fig 2.
Scanning electron microscopy observations of 48h-biofilm of wild-type EGD-e strain, DSS 1130 BFA2, EGD-eΔlmo2549 and EGD-eΔlmo2550 mutants on stainless steel at 30°C in MCDB202 medium.
Fig 3.
Cell surface characterization of L. monocytogenes with the hydrophobicity (Log K) (n = 3) and observations by transmission electron microscopy of wild-type EGD-e strain cells, DSS 1130 BFA2, EGD-eΔlmo2549, EGD-eΔlmo2550 mutants and the complemented mutants EGD-e Δlmo2549:: pLIV2(lmo2549) and EGD-e Δlmo2550:: pLIV2(lmo2550).
Fig 4.
Quantification and microscopic observation of populations from 48h biofilm of L. monocytogenes DSS 1130 BFA2, EGD-eΔlmo2549 and EGD-eΔlmo2550 mutants and wild-type EGD-e strain cultivated on stainless steel at 30°C in MCDB202 (A, B), after water flow (C, D), after NaOH flow (E, F).
The light gray of histograms indicates total populations, the gray indicates viable populations and the dark gray indicates viable culturable populations. The error bars represent the standard deviation (n = 6) and * represent significant difference.