Table 1.
Primers used for detecting potential pathogens and amplifying the complete genome sequences.
Table 2.
List of enterovirus strains used for phylogenetic analysis.
Fig 1.
Microphotograph showing cytopathic effects and viruses in Vero cells inoculated with processed fecal samples and spleen samples.
(A) Normal Vero cells; (B) Vero cells infected fecal specimen becomes rounded and detached from flask. C-D 20–30 nm virus particles were observed by electron microscope (EM) in the tissue culture infected with fecal specimen and spleen specimen (passage 5), indicated by white arrow. The scale bar is 100 nm.
Table 3.
Virus isolation or detection from samples of spleen and feces in goats.
Table 4.
Sequence identities of CEV-JL14 with known representative enterovirus species (A, B, C, D, E, F, G, H, J).
Fig 2.
CEV-JL14 belongs to a novel species within the genus.
Phylogenetic analysis of CEV-JL14 with representative EV-E, EV-F, EV-G and strains of other enterovirus species. (A) The phylogenetic tree generated by analyzing the complete genome sequence of CEV-JL14 with the representative EV-E, EV-F, EV-G and other enterovirus species. (B-C) Phylogenetic analyses of CEV-JL14 with the representative EV-E, EV-F, EV-G and strains of other species performed with the 5’-UTR, VP1 amino acid sequences by neighboring-joining methods. (D) Phylogenetic analysis of polyprotein sequence of CEV-JL14 with the representative EV-E, EV-F, EV-G and strains of other species. (E) Phylogenetic analysis of P1 of CEV-JL14 with the representative EV-E, EV-F, EV-G and strains of other species. The CEV-JL14 marked as solid triangle, together with TB4-OEV or other ovine/caprine enterovirus was clustered to a novel clade enterovirus L (EV-L). All the phylogenetic analyses above were performed using neighbor-joining method with a bootstrap value of 500.
Fig 3.
Phylogenetic analysis of CEV-JL14 with representative enteroviruses from other Enterovirus species.
Phylogenetic analysis of P1 (A), P2C (B), P3CD (C) and P2C+P3CD (D) of CEV-JL14 with the representative EV-E, EV-F, EV-G and strains of other species. The CEV-JL14 marked as solid triangle, together with TB4-OEV, CEV-JL14 was clustered to a novel clade enterovirus L (EV-L). All the phylogenetic analyses above were performed using neighbor-joining method with a bootstrap value of 500.