Fig 1.
MiR-93 expression in endometrial carcinoma tissues and normal endometrial specimens.
Expression levels of miR-93 in both endometrial carcinoma samples and normal samples were analyzed by RT-PCR (A). MiR-93 was highly expressed in EC tissue than in normal samples (B). *P < 0.05.
Fig 2.
MiR-93 transfection promoted endometrial carcinoma cell migration and invasion.
Following miR-93 transfection, miR-93 levels were significantly increased (A), endometrial carcinoma cell lines exhibited faster migration (B), and stronger invasion (C) compared with the control and mock cells 24 hours after transfection. Three representative microscopic fields were photographed to quantify the extent of invasion and migration, and three independent experiments were performed. Results are representative of three separate experiments; data are expressed as the mean ± standard deviation, *P < 0.05.
Fig 3.
MiR-93 transfection downregulated expression of E-cadherin and upregulated expression of the N-cadherin.
After miR-93 transfection, E-cadherin was downregulated and N-cadherin was upregulated in Western blot assay (A). Besides, Immunofluorescence assay was used to determine subcellular localization of E-cadherin and N- cadherin (B & C).
Fig 4.
MiR-93 transfection downregulated expression of FOXA1.
After miR-93 transfection the expression of RhoC had no significant differences (A) but FOXA1 was downregulated (D). According to a prediction website and Luciferase reporter assays demonstrated miR-93 could targets the 3’UTR of FOXA1 directly (B & C). MiR-93 inhibitor transfection induced FOXA1 expression (E).
Fig 5.
siFOXA1 transfection promoted endometrial carcinoma cell migration and invasion.
After siFOXA1 transfection, the expression levels of FOXA1 decreased significantly (A). Endometrial carcinoma cell lines exhibited faster migration (B), and stronger invasion (C) compared with the control and mock cells 24 hours after transfection. Three representative microscopic fields of each well (n = 3) were documented to quantify the extent of invasion and migration, and three independent experiments were performed. Data are expressed as the mean ± standard deviation, *P < 0.05.
Fig 6.
siFOXA1 transfection downregulated expression of E-cadherin and increased expression of the N-cadherin.
After siFOXA1 transfection, E-cadherin was downregulated and N-cadherin was upregulated in Western blot assay (A). Besides, Immunofluorescence assay was used to determine subcellular localization of E-cadherin and N- cadherin (B & C).