Fig 1.
The production of proteins versus their importance for growth.
(A) For each class of gene, we show the distribution of protein expression, in parts per million of amino acids (x axis, log scale). Proteins with little or no expression are shown at 0.1 ppm. (B) The aggregate expression of each class of gene.
Table 1.
The 20 most highly-expressed genes, by fraction of amino acids, that have no measurable impact on growth.
Fig 2.
The production of “unnecessary” proteins versus their importance in other conditions.
Only genes that were not important for fitness in minimal glucose media are included. The x axis is as in Fig 1A.
Fig 3.
Consistency of gene fitness values in minimal glucose medium.
(A) Consistency between replicates at 12 generations. Fitness values less than −3 are shown at −3 so as to focus on the more subtle fitness defects that are more susceptible to noise. 123 genes have fitness under −3 in both replicates. (B) Consistency across time. Genes with significant phenotypes (of either sign) are subdivided into those with weak expression (under 2 ppm of monomers) or above. Fitness values less than −3 are shown at −3. (C) Consistency within each gene. Fitness values at 12 generations were computed separately for the first and second half of each gene that had sufficient coverage. (D) shows the same data as (C), but only for fitness values above −3. In all panels, lines show x = 0, y = 0, and x = y.