Fig 1.
Location of selected regions within the mouse tibial mid-diaphyseal cross section.
(A) I-regions (Imax_A–anterior region around maximum second moment of area, Imax_P–posterior region around the maximum second moment of area, Imin_M–medial region around the minimum second moment of area, and Imin_L–lateral region around the minimum second moment of area) and (B) H-regions (P–periosteal, M–midcortical, E–endosteal; the section shows histological details for the periosteal, midcortical and endosteal regions selected within each I-region).
Fig 2.
The 3D-reconstructed model of lacuna and its canaliculi manually traced (violet lines) in the number of canaliculi analysis.
Table 1.
Descriptive statistics for the body and CSG properties between the Lc mutants and WT controls from the first data set.
Fig 3.
The mean of N.Ca (A, D), Lac.V (B, E) and lacunar orientation (C, F) by the I-regions and H-regions in the Lc mutants and WT controls from the second data set (Lc: open circles; WT: closed squares; vertical bars denote the 0.95 confidence intervals of the means; see abbreviations in Table 2).
Table 2.
Descriptive statistics for the number of canaliculi (N.Ca), lacunar volume (Lac.V) and orientation between the Lc mutants (n = 5) and WT controls (n = 5) from the second data set (letters in superscript indicate the Fisher’s LSD post-hoc significances between the I- and H-regions at p < 0.05).
Fig 4.
The distribution of N.Ca within a cross section of two Lc and two WT mice from the second data set (the color scale indicates the frequency of the N.Ca that range from the minimum (green) to maximum (red) frequencies; see abbreviation and units in Tables 1 and 2).
Fig 5.
The mean lacuna to lacuna distance by the (A) I-regions and (B) H-regions between the Lc mutants (open circles) and WT controls (closed squares) from the second data set. Vertical bars denote the 0.95 confidence intervals of the means.
Table 3.
Descriptive statistics for the lacuna to lacuna distance (Lac.Dist) between the Lc mutants (n = 5) and WT controls (n = 5) and the pooled mouse sample (letters in superscript indicate the Fisher’s LSD post-hoc significances between the I- and H-regions at p < 0.05).
Table 4.
Descriptive statistics for the number of canaliculi (N.Ca), lacunar volume (Lac.V) and orientation in the pooled mouse sample (letters in superscript indicate the Fisher’s LSD post-hoc significances between the I- and H-regions at p < 0.05).
Fig 6.
The mean lacuna to lacuna distance by the (A) I-regions and (B) H-regions between the Lc mutants (open circles) and WT controls (closed squares) from the second data set. Vertical bars denote the 0.95 confidence intervals of the means.