Table 1.
Primers used for qRT-PCR in this study.
Table 2.
Development index of zebrafish larvae after exposure to DEHP (0, 40, 100, 200, 400 μg/L) for 168 hpfa.
Fig 1.
The whole-body levels of thyroid hormone T4 (A), T3 (B) and the T4/T3 (C) of zebrafish larvae at 168 hpf were determined after exposure to 0, 40, 100, 200, 400 μg/L of DEHP.
The results were means ± SEM of three replicate samples. *P < 0.05 indicated significant difference between exposure groups and the control groups.
Fig 2.
The expressions of tshβ (A) and crh (B) and nis (C) of zebrafish larvae were detected by real-time PCR after exposure to 0, 40, 100, 200, 400 μg/L of DEHP at 168 hpf.
The results were means ± SEM of three replicate samples. Significant difference between exposure groups and the control groups was indicated by *P < 0.05 and **P < 0.01.
Fig 3.
The expressions of nkx2.1 (A) and pax8 (B) and tg (C) were measured by real-time PCR after exposure to 0, 40, 100, 200, 400 μg/L of DEHP at 168 hpf in zebrafish larvae.
The results were means ± SEM of three replicate samples. Significant difference between exposure groups and the control groups was indicated by *P < 0.05 and **P < 0.01 and ***P<0.001.
Fig 4.
The expressions of ugt1ab (A) and dio1 (B) and dio2 (C) were checked by real-time PCR after exposure to 0, 40, 100, 200, 400 μg/L of DEHP at 168 hpf in zebrafish larvae.
The results were means ± SEM of three replicate samples. Significant difference between exposure groups and the control groups was indicated by *P < 0.05 and **P < 0.01.
Fig 5.
The expression change of ttr (A) and trα (B) and trβ (C) were measured by real-time PCR, after exposure to 0, 40, 100, 200, 400 μg/L of DEHP at 168 hpf in zebrafish larvae.
The results were means ± SEM of three replicate samples. Significant difference between exposure groups and the control groups was indicated by *P < 0.05 and **P < 0.01 and ***P<0.001.