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Fig 1.

Renal cortical blood perfusion in Control, LVH, Control-H2S and LVH-H2S groups.

Data are expressed as mean± SEM. * represents P<0.05 compared to Control while # represents P<0.05 compared to LVH group (n = 6).

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Fig 1 Expand

Table 1.

The SBP, MAP heart index and LV index of control, LVH, control-H2S and LVH-H2S.

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Table 1 Expand

Fig 2.

(A, B, C and D). Data showing the concentration of H2S (A) NO (B), cGMP (C) in the kidney and H2S in urine (D) of Control, LVH, Control-H2S and LVH-H2S groups. Data are expressed as mean± SEM. * represents P<0.05 compared to Control while # represents P<0.05 compared to LVH group (n = 6).

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Fig 2 Expand

Fig 3.

(A, B, C, D and E). Data showing the expression of CSE mRNA (A), CBS mRNA (B), 3-MST mRNA (C), eNOS mRNA (D) and CSE activity (E) in the kidney of Control, LVH, Control-H2S and LVH-H2S groups. Data are expressed as mean± SEM. * represents P<0.05 compared to Control while # represents P<0.05 compared to LVH group (n = 9 in triplicate).

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Fig 3 Expand

Fig 4.

(A, B and C). Bar graph showing the overall mean of % drop in renal cortical blood perfusion in response to NA (A), PE (B) and ME (C) in Control, LVH, Control-H2S and LVH-H2S groups rats during saline, 5-MeU low dose and 5-MeU high dose phases. Values are mean± SEM of n = 6 rats in each group. * P<0.05 vs. Saline phase of same group and # P<0.05 vs. 5-MeU low dose phase of same group. ϕ P<0.05 vs. respective phase of Control and Ψ P<0.05 vs. respective phase of LVH groups.

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Fig 4 Expand

Fig 5.

(A, B and C). Dose response curve of renal vasoconstriction responses to set of doses of NA (A), PE (B) and ME (C) in Control, LVH, Control-H2S and LVH-H2S groups rats during saline phase, low dose phase and high dose phase of 5-MeU. Values are mean± SEM of n = 5–7 rats in each group. The significance is overall mean of 4 graded doses (each dose response is averaging the ascending and descending order responses) of an agonist in each phase and compared to saline phase and high dose phase. * P<0.05 vs. Saline phase and # P<0.05 vs. 5-MeU low dose phase.

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Fig 5 Expand

Fig 6.

(A, B and C). Bar graph showing the overall mean of % drop in renal cortical blood perfusion in response to NA (A), PE (B) and ME (C) in Control, LVH, Control-H2S and LVH-H2S groups rats during saline, CEC low dose and CEC high dose phases. Values are mean± SEM of n = 6 rats in each group. * P<0.05 vs. Saline phase of same group and # P<0.05 vs. CEC low dose phase of same group. ϕ P<0.05 vs. respective phase of Control and Ψ P<0.05 vs. respective phase of LVH groups.

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Fig 6 Expand

Fig 7.

(A, B and C). Dose response curve of renal vasoconstriction responses to set of doses of NA (A), PE (B) and ME (C) in Control, LVH, Control-H2S and LVH-H2S groups rats during saline phase, low dose phase and high dose phase of CEC. Values are mean± SEM of n = 5–7 rats in each group. The significance is overall mean of 4 graded doses (each dose response is averaging the ascending and descending order responses) of an agonist in each phase and compared to saline phase and high dose phase. * P<0.05 vs. Saline phase and # P<0.05 vs. CEC low dose phase.

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Fig 7 Expand

Fig 8.

Histopathological evidence of rat kidney of Control, LVH, Control-H2S and LVH-H2S groups using hematoxyllin and eosine staining.

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Fig 8 Expand

Fig 9.

Mechanism of action of hydrogen sulphide in resensitization of α1- adrenoreceptors by modifying the G-protein coupled 2nd messenger pathway.

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