Table 1.
CPDA-1 and MAP Composition.
Fig 1.
The viscosities of stored suspended red blood cells (SRBCs) from plateau (circle) and lowland (square) populations were measured throughout the 35-day storage period.
The viscosity at ahigh shear rate (A) (***t test p < 0.001 for plateau vs. lowland from day1 to day 35). The viscosity at low shear rate (B) (***t test p < 0.001 for plateau vs. lowland on day7, **t test p < 0.01 for plateau vs. lowland from day14 today 35).# ANOVA p < 0.05 for the viscosities of plateau SRBCs at high and low shear rates throughout the entire storage duration.
Table 2.
The results of RBC numbers and Hb concentrations in the lowland (n = 8) and plateau groups (n = 8) on storage day 1.
Table 3.
Hct and MCV in the lowland (n = 8) and plateau (n = 8) groups throughout the entire storage period.
Fig 2.
Standard quality parameters of stored suspended red blood cells (SRBCs) from plateau (circle) and lowland (square) populations were measured throughout the 35-day storage period.
pH (A) (***t test p < 0.001 for plateau vs. lowland on days1 and 35), **t test p < 0.01 for pl eau vs. lowland on days 7 and 14).Crystal osmolarity (B) (***t test p < 0.001 for plateau vs. lowland from day1 to day 35).Lactate concentration (C) (***t test p < 0.001 for plateau vs. lowland on Days 7 and Day 35).Supernatant glucose (D) and Na+ (E)concentrations(***t test p < 0.001 for plateau vs. lowland on days7, 14 and 35).Supernatant K+ levels(F) (***t test p < 0.001 for plateau vs. lowland on days 7 and 35).# ANOVA p < 0.05 for all of the parameters throughout the entire storage duration.
Table 4.
The change rates of Na+, K+, glucose and lactate in SRBCs throughout the storage period.
Fig 3.
Scanning electron microscopy of stored red blood cells (RBCs).
RBCs isolated from stored lowland RBCs on day1(A) andday35(C) and from stored plateau RBCs on day1(B) and day 35(D); magnification:2000×.
Fig 4.
The adenosine triphosphate (ATP) and2,3-diphosphoglycerate (2,3-DPG) levels and oxygen tension to attain 50% oxygen saturation of Hb (P50) of stored RBCs from plateau (square) and lowland (stripe) populations were measured throughout the 35-day storage period.
ATP (A) 2,3-DPG (B)(***t test p < 0.001 for plateau vs. lowland on day 1, **t test p < 0.01 for plateau vs. lowland on day 7). P50 (C)(**t test p < 0.01 for plateau vs. lowland on day 14).
Fig 5.
The osmotic fragility and hemolysis of stored suspended red blood cells (SRBCs) from plateau (circle) and lowland (square) populations were measured throughout the 35-day storage period.
Osmotic fragility (A) (***t test p < 0.001forplateau vs. lowland from day 1 to day35). Hemolysis (B) (**t test p < 0.01 for plateau vs. lowland on day1, ***t test p < 0.001 for plateau vs. lowland on day7).