Fig 1.
Unrooted UPGMA dendrogram based on the Dice distances matrix depicting the similarities between DGGE profiles of faecal samples collected from 5 different captive cheetahs.
Samples are represented by circles, coloured by animal and labelled with number of months passed since the start of sampling.
Table 1.
Distribution of discriminating band-classes between sample sets of zoos PL and OV.
Fig 2.
The mean Dice coefficients between samples binned into sampling intervals and depicted per cheetah.
Fig 3.
Moving-window analysis and rate of change (Δt) value of consecutive DGGE profiles per animal.
Variability between month x and month x-2 was calculated from a matrix of similarities for DGGE patterns, based on the Pearson correlation coefficient.
Fig 4.
Concentrations of Clostridium cluster I, XI, XIVa and Bifidobacterium spp. in faecal samples of 2 cheetahs from Zoo Parc Planckendael (PL) and 3 cheetahs from Zoo Parc Overloon (OV), assessed by real-time PCR.
Boxplots show median, interquartile range, sample minimum and maximum. The open circle indicates an outlier value for animal B2. *P<0.05, two-tailed Student’s t-test.
Fig 5.
Scatter plots displaying the correlation between log10 concentrations of bacterial groups quantified with real-time PCR in captive cheetah’s faecal samples.
r = Pearson correlation coefficient, *correlation significant at the 0.05 level, **correlation significant at the 0.01 level.
Fig 6.
Temporal variation of Clostridium clusters I, XI and XIVa in faecal samples of 5 captive cheetahs, assessed by real-time PCR.
Rate of change (Δt) value of consecutive DGGE profiles calculated per animal.