Fig 1.
SaePQRS regulates biofilm formation by S. aureus strain Newman.
(A) Stationary phase cultures of the indicated strains were diluted to an OD660 of 0.05 and inoculated into wells of a microtiter plate. The microtiter plate wells had been precoated with human plasma. After 16 h, biofilms were washed, fixed, and stained with crystal violet. The wells labeled “medium” indicates wells that contained sterile biofilm medium. (B) Inhibition of UAMS-1 biofilm formation by culture supernatants. Stationary phase culture supernatants of strain Newman and its derivatives were harvested, filter sterilized and added to microtiter plate wells preinoculated with S. aureus UAMS-1 suspended in biofilm medium. The source of each culture supernatant is listed to the right of the picture. The wells labeled “medium” indicates sterile TSB-0G medium was added in place of an exogenous culture supernatant. Plates were incubated and processed as described for A.
Fig 2.
The biofilm inhibitory factor is a heat stable protein.
Stationary phase culture supernatants of strain Newman (wt) and Newman ΔsaeRS were harvested, filter sterilized, and diluted into biofilm medium. Anti-biofilm activity of supernatants was tested against S. aureus strain UAMS-1. (A) Proteinase K treatment of culture supernatants. Supernatants from the indicated strains were incubated with proteinase K and Tris buffer, or buffer only (mock prtK), at 42°C for 1 hr and then 95°C for 10 minutes, before adding to biofilm medium. “None” indicates wells containing UAMS-1 but no culture supernatant. Wells labeled “medium” contained sterile biofilm medium. (B) Supernatants were incubated at the indicated temperatures for the indicated time periods before adding to wells containing UAMS-1 suspended in biofilm medium.
Fig 3.
saeRS regulates attachment but not dispersal.
(A) Stationary phase cultures of Newman and the ΔsaePQRS::kan strain were diluted into biofilm medium, inoculated into a 24-well plate and allowed to attach for 1 h at 37°C before washing and staining with crystal violet. (B) Supernatants from Newman and Newman ΔsaePQRS::kan were diluted into biofilm media and the attachment phenotype of strain UAMS-1 was tested with and without supernatant supplementation. (C) Newman (row 2) and Newman ΔsaePQRS::kan (row 3) culture supernatants were added at the indicated times following inoculation with UAMS-1. Wells in row 1 were supplemented with sterile medium rather than culture supernatant.
Fig 4.
Strain-dependent production of the biofilm inhibitory protein.
(A) Biofilm formation by strain USA300 FPR 3757 and derivatives. Wells were inoculated with the indicated USA 300 FBR 3757 derivatives. +saePQRSL and +saePQRSP indicate the presence of plasmids pCWSAE50 or pCWSAE51, respectively. (B) Anti-biofilm activity of USA300 derivatives. Culture supernatants from the strains listed to the right of the figure were tested for inhibition of biofilm formation by strain UAMS-1.
Fig 5.
The biofilm inhibitory protein limits biofilm in S. aureus but not S. epidermidis strains.
Culture supernatants from strain Newman or the ΔsaePQRS::kan mutant were added to biofilm medium and anti-biofilm activity was tested against the bacterial strains listed to the left of the figure. “Medium” indicates that sterile medium was added in place of culture supernatant.
Fig 6.
Confirmation of RNA-Seq data using RT-qPCR.
RNA was isolated from cultures of strain Newman (saeSP), CYL11481 (saeSL) and CYL11771 (ΔsaeRS) for use in RT-qPCR assays. Values for the saeSL (open bars) and ΔsaeRS (grey bars) strains are expressed relative to wild type Newman, which was assigned an arbitrary value of 1. All assays were performed with at least two RNA preparations obtained from separate cultures.
Fig 7.
Zymograms of S. aureus murein hydrolase activity.
Cell free extracts of strains Newman (wt), saeSL (CYL11481) and ΔsaeRS (CYL11771) were run on 10% acrylamide-SDS gels containing heat killed S. aureus RN4220 cells (leftmost panel) or heat killed Micrococcus luteus cells (middle panel). Numbers to the left of each gel indicate the molecular weights of size standards. Dark bands indicate regions of murein hydrolase activity. The large clear band migrating at approximately 60 kDa is presumably the Map protein, which is highly expressed in strain Newman. The rightmost panel is a Coomassie Blue-stained gel showing the same extracts used for the other panels. The large band migrating at approximately 60 kDa is presumably the Map protein.
Fig 8.
Extracellular (e) DNA in culture supernatants.
Culture supernatants from strain Newman and its derivatives were filter sterilized, extracted with phenol-chloroform and ethanol precipitated. Precipitates were suspended in H2O. The volumes of water were varied to compensate for slight differences in OD660 of the initial cultures. (A) Agarose gel of isolated eDNAs. (B) qPCR results. qPCR was performed with isolated eDNAs using oligonucleotide primers specific for the hu gene. Data are expressed relative to the amount of eDNA in the saeSL strain supernatant, which was arbitrarily assigned a value of 1. Data are means from two independent cultures of each strain. Δnuc indicates a nuclease deficient mutant of Newman. TSB indicates material isolated from sterile culture medium (TSB-0G).
Table 1.
Strains and Plasmids used in this study.