Fig 1.
Schematic representation of the genomic organisation of the CASR showing location of the six SNPs selected for analysis.
The CASR gene consists of 8 exons (1a, 1b, 2–7). The start (ATG) and stop (TGA) codons are in exons 2 and 7, respectively. Exons 1a, 1b, the 5’ portion of exon 2, and the 3’ portion of exon 7 are untranslated. The 3’ portion of exon 2, exons 3, 4, 5, 6 and the 5’ portion of exon 7, encode the extracellular domain (light grey), and the mid portion of exon 7 encodes the transmembrane (dark grey) and intracellular (black) domain. Three of the six SNPs are located in the CASR promoter region. rs115759455 and rs7652589 are located 13kbp upstream from the TATA box of promoter 1 (P1) and rs1501899 is located 4.6kbp downstream of promoter 2 (P2). The remaining three SNPs (rs1801725, rs1042636 and rs1801726) encoding single amino acid substitutions (A986S, R990G and Q1011E, respectively) are clustered in exon 7. The 5’ and 3’ untranslated regions are shown as white boxes. ECD, extracellular domain; TMD, transmembrane domain; ICD, intracellular domain.
Table 1.
Baseline clinical, biochemical and radiological characteristics of the Brussels Renal Transplant Cohort.
Table 2.
CASR polymorphism genotype and allele frequencies in the Brussels Renal Transplant Cohort.
Table 3.
Univariate analysis of associations between CASR SNP genotypes and risk factors for aortic and coronary artery calcification.
Table 4.
Univariate analysis of associations between CASR SNP genotypes and occurrence of arterial calcification or cardiovascular (CV) events.
Table 5.
Comparison of CASR SNP genotypes in patients with low and high levels of aortic calcification (AoC) and coronary artery calcification (CAC).
Table 6.
Significant determinants of serum glucose and phosphate concentrations.