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Table 1.

Deferred antagonism assay of different BLIS producing S. salivarius strains using different production media.

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Table 2.

API 20 STREP and API 50S. salivarius isolates.

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Table 2 Expand

Table 3.

Antibiotic disc sensitivities of S. salivarius isolates.

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Figure 1.

Subsystem feature counts of S. salivarius strains NU10 and YU10 detected by RAST.

No S. pyogenes virulence determinants were detected.

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Table 4.

Virulence assessment for S. salivarius strains YU10 and NU10.

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Figure 2.

Growth kinetics of S. salivarius strains grown aerobically in different media.

A: THB (BD), B: BHI (BD), C: M17 (Merck), D: MRS (Merck), E: YNS and F: PTNYSMES.

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Table 5.

Typical compositions for media used to cultivate S. salivarius.

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Table 6.

Variation of the pH values of S. salivarius cultures grown in different media after 22 hours of growth.

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Figure 3.

Purification and detection of salivaricin G32 produced by strain YU10 grown in PTNYSMES.

A: Cation exchange chromatography of the cell extract using SP FF column, B1: RP HPLC of the pooled active fractions of salivaricin G32 obtained from A, B2: second RP HPLC of the active fraction obtained by B1. C: MALDI-TOF (MS) analysis of the pure salivaricin G32.

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Figure 3 Expand

Table 7.

Inhibitory activity recovered from cell extracts and cell-free supernatants of S. salivarius cultures.

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Figure 4.

Detection of levan-sucrase enzyme produced by strain YU10 using ESI-LC-MS/MS analysis.

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