Table 1.
NaCl Minimal Inhibitory Concentration (MIC), Minimal Bactericidal Concentration (MBC) and Killing quotient (KQ) distributions for the 85 CF isolates and the 4 reference strains of P. aeruginosa.
Table 2.
Comparison of NaCl Minimal Inhibitory Concentration (MIC), Minimal Bactericidal Concentration (MBC) and Killing quotient (KQ) distributions for subgroups of P. aeruginosa CF isolates according to mucoid characteristic, antimicrobial susceptibility pattern, ability to form biofilm and patient under HTS treatment.
Figure 1.
Time-kill kinetics of different NaCl concentrations against P. aeruginosa isolate Pa71.
3% NaCl solution (orange line, filled triangles), 5% NaCl solution - corresponding to the MBC value (violet line, dash), 7% NaCl solution (red line, filled circles), and 10% NaCl solution (green line, filled squares). Control (blue line, filled lozenges) was not exposed to NaCl. Time-kill curves of 15% (not shown) and 10% NaCl solution were similar.
Figure 2.
Time-kill kinetics of NaCl against P.aeruginosa Pa1 according to bacterial phase of growth and incubation temperature.
Inoculum in stationary phase of growth (A) and in logarithmic phase of growth (B) for Pa1 (NaCl MBC: 7%). Bacterial suspension in NaCl at the MBC value were incubated at 37°C (green dotted line, open circles) or 4°C (red dotted line, filled circles). Controls (full lines) were not exposed to NaCl and were incubated at 37°C (green line, open circles) or 4°C (red line, filled circles). The horizontal dotted line indicates a 4-log reduction in viability. B: dotted green (open circles) and red (filled circles) lines are superposed.
Figure 3.
Effect of three sub-inhibitory concentrations of NaCl against P. aeruginosa CF isolate biofilm biomass formation.
Tested for 55 adherent CF isolates of P. aeruginosa with MIC values > 3% distributed as follows: strongly adherent (n = 13, violet bars), moderately adherent (n = 15, green bars), weakly adherent phenotype (n = 27, blue bars). Prevention of biofilm biomass formation is presented as percentage of strains for which ability to form biofilm biomass was decreased by at least 25% compared to controls (not exposed).
Figure 4.
Activity of 7% and 10% NaCl solution against preformed biofilm for three strongly adherent isolates.
7% (green bars) and 10% (violet bars) NaCl solution was tested against preformed biofilm for the three P. aeruginosa CF isolates Pa28, Pa29, and Pa31. Results are expressed as percentage of biofilm viability compared to non-exposed control after 24 h of NaCl exposure.
Figure 5.
Time-kill kinetics of 10% NaCl solution against preformed biofilm for three strongly adherent CF isolates.
P. aeruginosa CF isolates: Pa28 (green line, filled circles), Pa29 (blue line, filled squares), Pa31 (violet line, filled lozenges).
Figure 6.
Rate of motile isolates according to NaCl concentration.
Green bars show the number of motile isolates among isolates with non-inhibited growth (full bars).