Figure 1.
Hybrid crosses and an example of AFLP gel showing bands of parental species and hybrids.
A) Interspecific cross and backcrosses used in experiments B) Selective PCR AFLP band patterns using primers with selective nucleotides GG (EcoRI) and CTG (MseI). The arrows indicate two instability markers detected in hybrids from backcross 1 as an example. MWM, molecular weight marker; Dk, D. koepferae; Db, D. buzzatii; HF1, F1 hybrids; BC1, backcross 1.
Table 1.
Comparison of the number of instability markers in backcross 3 (BC3) between hybrid families.
Figure 2.
Markers in hybrids and parental species.
Table 2.
Sequences of AFLP markers showing homology to TE sequences.
Figure 3.
Percentage of transposition markers in hybrid.
Table 3.
Comparison of the number of transposition markers between BC3 hybrid families and parental species.
Table 4.
Chromosomal insertion sites of Osvaldo, Helena and Galileo in parental species.
Table 5.
Number of Osvaldo, Helena and Galileo insertions detected by transposon display.
Table 6.
Transposition rates of Osvaldo, Helena and Galileo in hybrid families and parental species.
Table 7.
Comparison of the number of new insertions between hybrids and parental species by Mann-Whitney test.
Table 8.
Comparison of the number of new insertions between hybrid families by Kruskal-Wallis test.