Table 1.
3′-RACE cloning of candidate reference genes and cold-inducible genes.
Figure 1.
Distribution of threshold cycle (Ct) of tested reference gene regions.
Lower line, lower box, upper box and upper line indicate percentiles of up to 25, 50, 75 and 100% range, respectively.
Table 2.
Ratio (mean and standard deviation) of initial amount of cDNA templates between CDS and 3′-UTR primer regions in the same gene.
Figure 2.
Parameters calculated from geNorm analysis.
(A) Average expression stability value M for best performed 5, 4 or 3 genes. (B) Pair-wise variation of normalization factors for best performing 3 or 4 genes (V3/4). Group symbols are as in Table 6.
Table 3.
Stability ranking of reference gene regions evaluated by geNorm.
Figure 3.
Stability value calculated from NormFinder.
Table 4.
Stability ranking of reference gene regions evaluated by NormFinder.
Table 5.
Stability ranking of reference gene regions evaluated by ΔCt.
Figure 4.
Normalized expression level of PpCBF2 and PpCBF4.
(A) Normalization by best stable genes for overall samples (HIS_cds, SAND_utr, TIP_utr). (B) By least stable genes for overall samples (bTUB_utr3, ACT_utr2, bTUB_utr2). (C) By best stable genes for FB (bTUB_utr3, HIS_utr2, ANX_utr). (D) By least stable genes for FB (EF1a_cds, GAP_utr2, bTUB_utr2).
Table 6.
A descriptive list of the different samples used in this study.