Table 1.
Study setup.
Figure 1.
Regions of the mouse brain where tissueimmunohistochemically analyzed for content of myelin (PLP) and oligodendrocytes (NOGO-A) were analyzed.
Figure 2.
Longitudinal MTR values fitted with LMER.
LMER regression of the difference in MTR values between cuprizone exposed animals and control animals over time. Dots represent single measures and the gray shaded area standard deviations of the LMER fit. A: The corpus callosum. B: The deep gray matter. C: The olfactory bulb. D: The cerebellum. E: The cerebral cortex.
Table 2.
LMER model parameters.
Figure 3.
Progression of myelin content over time, estimated from PLP stained tissue. Lateral in the corpus callosum (red ), medial in the corpus callosum (green
), in the deep gray matter (blue
) and in the cerebral cortex (black
).
Figure 4.
Progression of oligodendrocyte density over time, estimated from NOGO-A stained tissue. Lateral in the corpus callosum (red ), medial in the corpus callosum (green
), in the deep gray matter (blue
) and in the cerebral cortex (black
).
Figure 5.
PLP area correlated to MTR value.
Correlation between myelin content, estimated from PLP stained tissue, and MTR values. Dots represent animals, with MTR measurement taken imediately before being sacrificed for histological staining.
Figure 6.
NOGO-A count correlated to MTR value.
Correlation between oligodendrocyte density, estimated from NOGO-A stained tissue, and MTR values. Dots represent animals, with MTR measurement taken imediately before the animal was sacrificed for histological staining.
Figure 7.
Myelin stained histology and MTR images.
Representative images of PLP stained histology, from the cerebral cortex, the deep gray matter and the corpus callosum, and MTR image at baseline, 1,3 and 6 weeks of cuprizone exposure and two weeks after exposure end. Notice visible decrease of MTR values in the corpus callosum, corresponding to decrease seen in the PLP stained images.
Table 3.
PLP - MTR correlation parameters.
Table 4.
NOGO-A - MTR correlation parameters.