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Figure 1.

Individualized splint of the lower arch.

A) Parts of the individualized splint: 1. internal vinyl sheet; 2. polished glass discs; 3. fenestrated external vinyl sheet. B) Clinical view of the individualized splint with the glass discs inserted (arrows).

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Figure 1 Expand

Figure 2.

Mean percentages of bacterial viability in saliva and de novo PL-biofilm under basal conditions and at different times after a single mouthrinse of sterile water and 0.2% Chlorhexidine.

A) Intra-mouthrinse differences; B) Inter-mouthrinse differences; C) Inter-ecosystem differences.

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Figure 2 Expand

Table 1.

Mean percentages of bacterial viability in PL-biofilm, as well as intra-mouthrinse and inter-mouthrinse comparisons.

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Table 1 Expand

Table 2.

Mean values of thickness (µm) in PL-biofilm, as well as the intra-treatment and inter-treatment comparisons.

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Table 2 Expand

Figure 3.

Images representing the changes in bacterial viability in the saliva.

A) Sample collected under basal conditions; B), C), D) Sample collected at 30 seconds, 5 and 7 hours respectively after a single mouthrinse of 0.2% Chlorhexidine.

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Figure 4.

Images representing the changes in bacterial viability in de novo PL-biofilm.

A) Sample collected under basal conditions; B), C), D) Sample collected at 30 seconds, 3 and 7 hours respectively after a single mouthrinse of 0.2% Chlorhexidine.

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Figure 5.

Visualization of channels (A, yellow star) and voids (B, white star) in a single cross sectional plane from X, Y and Z axis images obtained by the CLSM from a baseline sample.

The presence of both channels and voids shows a heterogeneous architecture model.

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