Table 1.
Overview of cases included for comparing mitoses counts on glass slides and whole slide images.
Figure 1.
Snapshot of WSI showing the annotated areas for scoring mitosis microscopically and digitally.
1- An overview image of the WSI, 2- The defined area for counting mitosis microscopically, 3- The defined area for counting mitosis digitally, 4a- Microscopic snapshots of different mitotic figures, 4b- Digital snapshots of different mitotic figures.
Figure 2.
Snapshots from several WSI of several breast resections diagnosed previously as an infiltrative ductal carcinoma using a light microscopy.
These snapshots showing different appearances of mitotic figures encircled by green circles. Panels A–C show cells in early metaphase. Panels D–G show different forms of mitotic division in late metaphase. Panel H–L shows different forms of anaphase. Panels M–P show cells in telophase.
Figure 3.
Snapshots of WSI showing the possible appearance of mitosis-like figures surrounded by green circles.
Figure 4.
Overview of stepwise kappa statistics between observers.
Figure 5.
Scatter plots of microscopic versus digital MAI counts per observer indicating the tendency to underestimate of mitotic counts on WSI.
Table 2.
Overview of mitotic counts showing the trends towards underestimating mitotic counts when performed digitally.
Table 3.
Concordance rate of each observer for scoring Mitotic Activity Index (MAI) using WSI and conventional microscopy.