Table 1.
Ingredients and chemical composition of diets used in this study.
Table 2.
Primer sequences used for real-time PCR analysis.
Figure 1.
Zinc concentration in the liver of piglets fed LZn (50 mg/kg), NZn (150 mg/kg), or HZn (2500 mg/kg) levels of dietary zinc.
abdifferent superscripts indicate significant (P<0.05) differences.
Figure 2.
Effects of dietary zinc supplementation on metallothionein expression.
Relative metallothionein mRNA levels (A) and protein expression (B) in the liver of piglets fed LZn (50 mg/kg), NZn (150 mg/kg), or HZn (2500 mg/kg) diet.
Figure 3.
Effects of dietary zinc supplementation on proteomic proteomic profile of porcine liver.
Representative 2D gel containing liver proteins from a pool of all analyzed liver samples stained with Cy2. Marked spots represent differentially expressed proteins in hepatic tissue comparing HZn (spot 1–17) or LZn fed piglets (spot 3A, 18A, 19A) with those receiving NZn diet and correspond to the respective spot numbers found in Tables 3 and 4.
Table 3.
List of identified proteins in hepatic tissue of piglets showing differential expression in response to various dietary zinc concentrations.
Table 4.
Function of differentially expressed proteins.
Figure 4.
Effects of dietary zinc supplementation on relative mRNA level of differentially expressed liver proteins.
Relative mRNA level of HSP70 (A), SERPINH1 (B), APOA1 (C), ARG1 (D), AIFM1 (E), and EIF4A1 (F) in hepatic tissue of piglets fed LZn (50 mg/kg), NZn (150 mg/kg), or HZn (2500 mg/kg) levels of dietary zinc. Different superscripts indicate significant (P<0.05) differences.
Figure 5.
Validation of differential protein expression by Western blotting.
Western blot demonstration of differentially expressed proteins TRFE (A), ARG1(B), and HSP70(C) in the liver of piglets fed NZn (150 mg/kg), or HZn (2500 mg/kg) diets.