Figure 1.
Total and active bacteria found in feces of children.
Relative abundance (%) of total bacterial composition (16S-rDNA) (A) and metabolic active bacteria (16S-rRNA) (B) at the phylum level found in the fecal samples of Pervasive Developmental Disorder Not Otherwise Specified (PDD-NOS), autistic (AD) and healthy (HC) children.
Figure 2.
Principal component analysis (PCA) of active bacteria genera found in feces of children.
Score plot of the three principal components (PC) after PCA of the total bacterial genera information (16S-rRNA) for Pervasive Developmental Disorder Not Otherwise Specified (PDD-NOS), autistic (AD) and healthy (HC) children. 1-10, number of fecal samples for each group of children.
Figure 3.
Permutation analysis of the active bacterial genera found in feces of children.
Metabolically active bacterial genera composition found in Pervasive Developmental Disorder Not Otherwise Specified (PDD-NOS), autistic (AD) and healthy (HC) children. Double dendrogram representation of clustering was performed. The genera that showed values less than 0.1% of the total metabolically active bacterial were grouped together on the same phylum (others Actinobacteria, others Bacteroidetes, others Firmicutes, others Proteobacteria). 1-10, number of fecal samples for each group of children.
Figure 4.
Fecal levels of free amino acids (FAA) in children.
Concentration (mg/kg) of FAA found in fecal samples of Pervasive Developmental Disorder Not Otherwise Specified (PDD-NOS), autistic (AD) and healthy (HC) children. Data are the means of three independent experiments and standard deviations, performed in duplicate (n=6).
Figure 5.
Canonical Discriminant Analysis of Principal Coordinates (CAP) of volatile organic metabolites in feces of children.
Loading coefficient plots of the volatile organic compounds from fecal samples of Pervasive Developmental Disorder Not Otherwise Specified (PDD-NOS) (A), autistic (AD) (B) and healthy (HC) children. Compounds significantly associated with the feces of PDD-NOS children (negative axis) or HC children (positive axis) Data are the means of three independent experiments (n = 3).
Figure 6.
Principal component analysis (PCA) of volatile organic metabolites found in feces of children.
Score plots of the two principal components (PC) after PCA of volatile organic metabolites found in fecal samples of Pervasive Developmental Disorder Not Otherwise Specified (PDD-NOS), autistic (AD) and healthy (HC) children. All the variables used were listed in Table S1.