Table 1.
Basal plasma electrolyte and hormones and basal cardiovascular and renal parameters in 5 year old sham and uninephrectomised (uni-x) sheep.
Figure 1.
Cardiovascular and renal responses to intravenous infusion of low pressor dose angiotensin II (AngII) expressed as percentage change (%Δ) from basal period 1 in sham and uni-x female sheep at 5 years of age (n = 5/group).
MAP, mean arterial pressure; RVR, renal vascular resistance; RBF, renal blood flow; GFR, glomerular filtration rate; UFR, urine flow rate; UNaV, urinary sodium excretion; all values except MAP are corrected for total kidney weight (gkw). Sham: white bars, uni-x: black bars, *P<0.05, ***P<0.001, ****P<0.0001 from Bonferroni post-hoc test comparing responses of sham or uni-x animals to AngII infusion from their basal period 1. #P<0.05, ##P<0.01, ###P<0.001 from student’s t-test comparing sham and uni-x. Values are mean ± SEM.
Figure 2.
Cardiovascular and renal responses to AT1R blockade via intravenous infusion of losartan expressed as percentage change (%Δ) from basal period 2 in sham and uni-x female sheep at 5 years of age (n = 5/group).
MAP, mean arterial pressure; RVR, renal vascular resistance; RBF, renal blood flow; GFR, glomerular filtration rate; UFR, urine flow rate; UNaV, urinary sodium excretion; all values except MAP are corrected for total kidney weight (gkw).B2, basal period 2; sham: white bars, uni-x: black bars, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 from Bonferroni post-hoc test comparing responses of sham or uni-x animals to losartan from their basal period 2. #P<0.05, ##P<0.01, ###P<0.001, ####P<0.0001 from student’s t-test comparing sham and uni-x.Values are mean ± SEM.
Figure 3.
Cardiovascular and renal responses to Angiotensin II infusion in the presence of AT1R blockade via losartan expressed as percentage change (%Δ) from losartan infusion period in sham and uni-x female sheep at 5 years of age (n = 5/group).
MAP, mean arterial pressure; RVR, renal vascular resistance; RBF, renal blood flow; GFR, glomerular filtration rate; UFR, urine flow rate; UNaV, urinary sodium excretion; all values except MAP are corrected for total kidney weight (gkw). Sham: white bars, uni-x: black bars, *P<0.05, **P<0.01, ***P<0.001, from Bonferroni post-hoc test comparing responses of sham or uni-x animals to AngII plus losartan from their losartan infusion period. Values are mean ± SEM.
Figure 4.
Angiotensin II receptor gene expression in the kidney cortex and medulla of 5 year old female sheep (A) Angiotensin II receptor type 1 (AT1R), (B) Angiotensin II receptor type 2 (AT2R).
Sham (white bars, N = 5) and uni-x (black bars, N = 5) Values are mean ± SEM. #P<0.05 comparing sham and uni-x animals and *P<0.05 comparing kidney zones within the sham or uni-x group from Bonferroni post-hoc test.
Figure 5.
Renal tissue (A) renin content, angiotensin peptide (Angiotensin II (AngII), Angiotensin 1–7 (Ang 1–7) content in the renal (B) cortex and (C) medulla, and (C) Angiotensin 1–7:AngII ratio in sham and uni-x kidneys at 5 years of age.
Sham (white bars, N = 5) and uni-x (black bars, N = 5). Values are mean ± SEM. #P<0.05, ##P<0.01, ###P<0.001 comparing sham and uni-x groups and *P<0.05, **P<0.01, comparing kidney zones within the sham and uni-x group from Bonferroni post-hoc test.