Figure 1.
Structure of a mature seed of ultrahigh oil content B. napus line YN171.
(A) Entire seed, showing the seedcoat, radicle and mature cotyledons. Bar = 200 µm. (B) Ultrastructure of the seedcoat, showing the epidermis (ep), palisade (pal) and aleuronic layer (al). (C) Ultrastructure of the aleuronic cell, the local amplification of the white frame part in (B), showing the protein bodies (arrowheads) and oilbodies (arrows). (D) Ultrastructure of the outer radicle cell, showing the protein bodies (arrowheads) and oilbodies (arrows). (E) Ultrastructure of the cotyledon cell, showing the protein bodies (arrowheads) and oilbodies (arrows). Bars are 200 µm in (A), 10 µm in (B), 1 µm in (C) and 5 µm in (D) and (E).
Table 1.
The traits average values of B. napus seeds.
Figure 2.
Seed morphology of 7 rapeseed lines with different oil content.
The upper is the ultrahigh oil content line YN171, the middle is three high oil content lines ZY036, 6F313 and 09–88, the nether is three low oil content lines 51070, 7ml177-3 and 9Q629-3. Bar is 1 cm.
Figure 3.
TEM analysis of 7 lines different oil content rapeseed mature cotyledons.
The upper is the ultrahigh oil content line YN171 with three observations (YN171-1, YN171-2 and YN171-3), the middle is three high oil content lines ZY036, 6F313 and 09–88, the nether is three low oil content lines 51070, 7ml177-3 and 9Q629-3. o, oil body; p, protein body; Bar is 2 µm.
Figure 4.
TEM analysis of 7 lines different oil content rapeseed seedcoats.
(A) Ultrastructure of seedcoat, showing the epidermis (ep), palisade (pal) and aleuronic layer (al). From left to right, YN171, ZY036, 6F313, 09–88, 51070, 7ml177-3 and 9Q629-3. (B) Ultrastructure of aleuronic cell in rapeseed seedcoat, showing the oilbodies (o) and protein bodies (p). The upper is the ultrahigh oil content line YN171 with three observations (YN171-1, YN171-2 and YN171-3), the middle is three high oil content lines ZY036, 6F313 and 09–88, the nether is three low oil content lines 51070, 7ml177-3 and 9Q629-3. Bars are 10 µm in (A) and 5 µm in (B).
Figure 5.
TEM analysis of 7 lines different oil content rapeseed radicles.
The upper is the ultrahigh oil content line YN171 with three observations (YN171-1, YN171-2 and YN171-3), the middle is three high oil content lines ZY036, 6F313 and 09–88, the nether is three low oil content lines 51070, 7ml177-3 and 9Q629-3. o, oil body; p, protein body; Bar is 5 µm.
Figure 6.
Fatty acid profiles of B. napus seeds.
Table 2.
Correlation coefficients of seed oil content, protein content, seedcoat ratio, cotyledon ratio, radicle ratio, and 1000-seed weight of 68 B. napus lines.