Figure 1.
Expression patterns of BnTT10, BrTT10 and BoTT10 genes in black- and yellow-seeded lines.
QRT-PCR detection of BnTT10 (A), BrTT10 (C) and BoTT10 (E) family mRNA, and transcript levels of BnTT10 (B), BrTT10 (D) and BoTT10 (F) family members in reproductive organs of B. napus, B. rapa and B. oleracea black- and yellow-seeded near-isogenic lines. Error bars represent standard deviations (n = 3 for A, C and E).
Figure 2.
Seed pigmentation observation.
Seedpods were sampled at 42, 50, 55, 60 and 45 DAF and the opened pods were observed under a low-power stereoscope. 5 DAH: five days after harvest. Seed coat pigmentation in the T2 transgenic and control B. napus cv. Zhongyou821 (A) and Zhongshuang10 (B).
Figure 3.
Soluble and insoluble PAs measured after acid-catalyzed hydrolysis in seed coats transgenic and control lines.
The P-value is for a t-test for means of paired samples. W-10, W-12, W-13: transgenic lines with inhibited BnTT10 expression; W-22: transgenic lines with no inhibition in BnTT10 expression; W-24: control lines with normal BnTT10 expression. T2-P: positive T2 progenies; T2-N: negative T2 progenies after separation. Soluble (A) and insoluble PA (B) content in seed coats of T2 transgenic and control B. napus cv. Westar plants. Each value represents the means of three independent experiments +/− SD.
Figure 4.
Lignin content in seed coats of transgenic and control lines.
Lignin content was analyzed for seed coats of T2 antisense BnTT10 transgenic and control lines using the acetyl bromide method. Data are means for three T2 progenies of each line, with triplicate measurements in each sample. The P-value is for a t-test for means of paired samples. W-10, W-12 and W-13: transgenic lines with inhibited BnTT10 expression; W-22: transgenic lines with no inhibition in BnTT10 expression; W-24: control lines with normal BnTT10 expression. T2-P: positive T2 progenies; T2-N: negative T2 progenies after separation. Each value represents the means of three independent experiments +/− SD.
Figure 5.
Detection of flavonoid composition in seed coats from transgenic and control B. napus plants.
Analyses were performed by LC-UV-MS on seed coats of T2 antisense BnTT10 transgenic and control lines of B. napus cv. Zhongyou821. Q-3-G, Quercetin-3-glucoside; PC dimer B2, [DP2]-B2, epicatechin-(4β-8)-epicatechin; EC, epicatechin; K-3-O-G-7-O-G, kaempferol-3-O-glucoside-7-O-glucoside; I-di-H, isorhamnetin-dihexoside. Each value represents the means of three independent experiments +/− SD.