Table 1.
Body weight, BMD, Uterine index, and Serum estradiol from the OVX and the Sham rats.
Figure 1.
Biochemical parameters in serum.
Biochemical parameters in serum from the Sham group and the OVX group over the whole experiment period. The values represent as the Means±SD. N = 8. (*P<0.05, **P<0.01 vs Sham).
Figure 2.
Typical GC-TOF/MS total ion chromatograms of rat plasma.
Typical GC-TOF/MS total ion chromatograms of rat plasma obtained from the Sham group (A) and the OVX group (B).
Figure 3.
PLS-DA score plots and 3D plots.
Partial least squares projection to latent structures and discriminant analysis (PLS-DA) score plots (A: PC1/PC2) and 3D plots (B: PC1/PC2/PC3) of the OVX group over the process of bone loss. X-axis, Y-axis and Z- axis were labeled with PC1 (the first principal component), PC2 (the second principal component), PC3 (the third principal component) respectively. Letters M denotes the OVX group. Numbers 0–12 represents different time points during the experiment: 0, before experiment; 3, week 3; 6, week 6 et al. M3 means the point from the sample in the OVX group at the 3rd week after the experiment. One data point stands for one subject: for instance, M3 and M6 mean the point from the sample in the OVX control rats at the 3rd and 6th week after surgery.
Figure 4.
Kinetics of metabolic changes in the OVX plasma.
Kinetics of metabolic changes in the OVX plasma over the 12 week post operation.
Figure 5.
PLS-DA score plots of plasma samples.
(A) PLS-DA score plots of OVX rats and sham rats at the 12th weeks post-surgery; (B) PLS-DA score plots of OVX rats and sham rats at the 24th weeks post-surgery; (C) PLS-DA score plots of OVX rats between the 12th weeks and 24th week post surgery. Letters M and S denotes the OVX group and the Sham group, respectively. Numbers 12 and 24 represents different time points during the experiment: 12, week 12; 24, week 24.
Figure 6.
Relative changes of plasma metabolite levels.
Relative changes of plasma metabolite levels in the sham group versus the OVX group at the 12th weeks and 24th weeks post surgery.
Table 2.
Fold changes and P value of endogenous metabolites detected by GC/TOF-MS.