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Figure 1.

Genome organization of MXPyV.

The 4,939-nt circular genome of MXPyV (A) contains putative coding regions for VP1, VP2, VP3, ST-Ag, and LT-Ag (yellow arrows). C1, C2, and C3 (gray) denote de novo assembled contigs from deep sequencing data. (B) Domains and binding motifs present in the spliced LT-Ag and ST-Ag of MXPyV.

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Figure 2.

Amino acid phylogenetic analysis of MXPyV relative to other polyomaviruses.

(A) VP1, (B) VP2, (C) ST-Ag, (D) LT-Ag. Bayesian support levels are indicated at each branching point. Abbreviations: AGM, African green monkey; SV40, simian virus 40; SV12, simian virus 12; SqMPy, squirrel monkey; CaliSeaLion, California sea lion. Other abbreviations and GenBank accession numbers are described in the text. Note that Merkel cell virus (MCV) is not included in the LT-Ag phylogeny due to the presence of truncation mutations.

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Figure 3.

Amino acid identities of the VP1, VP2, small T-antigen, large T-antigen of MXPyV relative to that of other polyomaviruses.

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Table 1.

Results from MXPyV screening of clinical samples by RT-PCR.

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Table 2.

Comparison of quantitative RT-PCR vs. PCR assays for detection of MXPyV and titers of MXPyV in stool.

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Table 3.

Gastroenteritis symptoms corresponding to MXPyV-positive stool samples compared to uninfected samples in the California SIFT study.

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Table 4.

Demographics of individuals who provided stool samples from the California SIFT study according to MXPyV positivity.

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Figure 4.

Whole-genome sequence alignment of MXPyV relative to other recently described gut-associated polyomaviruses HPyV10 and MWPyV.

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