Figure 1.
Microfluidic impedance cytometer system.
Microfluidic impedance cytometer (A) front-end electronics board mounted with impedance chip (B) close-up (top view) of chip showing two pairs of overlapping electrodes above and below micro-channel (C) schematic diagram (side view) showing detection electronics and cell/platelet inside micro-channel.
Figure 2.
Concordance between venous and fingerprick samples on standard hematology analyzer.
Concordance between venous and fingerprick blood samples for total and 3-part differential leukocyte concentrations measured using a Sysmex XE-2100 hematology analyzer (n = 36). Solid line shows linear least-squares regression of all leukocyte populations.
Figure 3.
Bland-Altman analysis of venous/fingerprick concordance.
Bland-Altman plots showing mean of against difference between venous and fingerprick blood samples for (A) Total WBC (B) Granulocyte (C) Lymphocyte and (D) Monocyte concentrations measured using the Sysmex XE-2100 (n = 36). Thick black line shows bias (average difference) between venous and fingerprick, dashed (colored) lines show the 95% limits of agreement (LOA), (average difference ±1.96 SD).
Table 1.
Bland-Altman parameters for venous/fingerprick concordance on standard analyzer.
Figure 4.
MIC impedance data measurement of venous sample.
Impedance data obtained from MIC measurement of a typical venous blood sample after red cell lysis plotted as electrical cell volume, Φ, against opacity, O, displayed as (A) intensity scatter plot (color indicates cell number according to color bar) and (B) scatter plot showing manual gates (black polygons) around the three main leukocyte populations to obtain granulocyte (red), lymphocyte (blue) and monocyte (green) counts.
Figure 5.
Concordance between same-site samples measured on standard analyzer and MIC.
Concordance between Sysmex XE-2100 hematology analyzer and MIC for (A) venous (n = 9) and (B) fingerprick (n = 9) blood samples for total and 3-part differential leukocyte concentrations. Solid black lines show linear least-squares regression to all leukocyte populations, with first-order and correlation coefficients stated.
Table 2.
Bland-Altman parameters for concordance between venous samples measured on standard analyzer and MIC.
Table 3.
Bland-Altman parameters for concordance between fingerprick samples measured on standard analyzer and MIC.