Table 1.
Sequence and assembly summary of B.tabaci transcriptome.
Figure 1.
Top BLAST hits of B. tabaci sequences to various insect species.
Figure 2.
Distribution of unigenes based on the Gene Ontology (GO) functional categories.
Table 2.
Summary of microsatellite loci predicted in B.tabaci transcriptome.
Figure 3.
Diversity and phylogeny of bacterial symbionts in B. tabaci.
Phylogenetic relationships of symbiotic bacteria in B.tabaci were resolved at the Genus levels.
Table 3.
Genes of interest in B.tabaci transcriptome.
Figure 4.
COG classification of the symbiotic bacteria in B. tabaci.
ME: Metabolism; CPS: Cellular processes and signaling; ISP: Information storage and processing; PC: Poorly characterized (inside circle); Q: Secondary metabolites biosynthesis, transport and catabolism; F: Nucleotide transport and metabolism; H: Coenzyme transport and metabolism; I: Lipid transport and metabolism; G: Carbohydrate transport and metabolism; C: Energy production and conversion; P: Inorganic ion transport and metabolism;E: Amino acid transport and metabolism; N: Cell motility; D: Cell cycle control, cell division, chromosome partitioning; U: Intracellular trafficking, secretion, and vesicular transport; V: Defense mechanisms; O: Posttranslational modification, protein turnover, chaperones; T: Signal transduction mechanisms; M: Cell wall/membrane/envelope biogenesis; J: Translation, ribosomal structure and biogenesis; L: Replication, recombination and repair; K: Transcription; S: Function unknown; R: General function prediction only.
Figure 5.
Schematic drawing of amino-acid biosynthetic pathways in B.tabaci.
Essential (A) and non-essential (B) amino acid biosynthetic pathways were deduced from B.tabaci transcriptome sequences. The sequential pathways are represented by boxes which indicate one step catalyzed by the named enzyme. Blue box denotes the enzyme detected exclusively in the bacterial group, red box represents the enzyme only detected in the insect group, and purple box designates for the enzyme found in both groups. Outside the boxes, the three-letter abbreviations of amino acids are highlighted in black; compounds detected only in the insect group are in red; compounds found only in the bacterial group are in blue; and compounds detected in both groups are in purple.
Table 4.
Putative P450s in B. tabaci transcriptome.
Figure 6.
Gene expression profiling of putative P450s in thiamethoxam resistant and susceptible B. tabaci.
mRNA expression levels of P4506a8 (A), CYP4v2 (B), and CYP6v5 (C) in thiamethoxam resistant (TH-2000) and susceptible (TH-S) B. tabaci were analyzed by the qRT-PCR. Relative gene expressions in the resistant TH-2000 population were normalized to the susceptible TH-S population using both 18s rRNA and β-actin as the internal references. Standard errors were generated from the three biological replicates.
Table 5.
Current available whitefly transcriptomes.