Table 1.
Genetics of FusE class mutants.
Table 2.
Growth compensated mutants of FusA SCVs.
Table 3.
Growth compensated mutants of a FusA menadione-auxotrophic SCV.
Table 4.
Selection of growth-compensated mutants from FusE L6 SCVs.
Table 5.
Selection of growth-compensated mutants from FusE Hemin and Menadione-auxotrophic SCVs.
Figure 1.
The affect of the addition of hemin or menadione to the growth medium (Mueller-Hinton Broth or agar) on MIC KAN (part A) and growth rate (part B) for isogenic strain pairs (SCVs and growth-compensated mutants).
AH001 is the wild-type strain. Results shown are the median value of six to ten independent measurements for MIC measured by Etest, and the mean of two independent measurements of growth rate measured in a Bioscreen C machine.
Figure 2.
Illustration of a FusE L6-Men SCV derived from S. aureus 8325-4, and how it was evolved back to normal growth by selecting for faster-growing mutants in two steps (clockwise).
A. S. aureus AH001 (WT), normal growth; B. AH386, FusE L6-Men SCV (rplF, menB); C. AH483 (rplF, menB WT); D. AH562 normal growth (rplF WT, menB WT).
Table 6.
Selection of growth-compensated mutants from an SCV in two steps.