A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues
Fig 1
Three examples of nuclear dissociation trials in Atlantic salmon liver at x40 magnification, stained with trypan blue.
A. Nuclei did not spend sufficient time in the dissociation buffer resulting in incomplete dissociation. This may result in the obstruction of the microfluidic device and failure of the library. B. Nuclei perfectly dissociated showing minimal aggregation, ideal for library preparation. C. Nuclei have been in the dissociation buffer for too long, resulting in degradation of the nuclear membrane, which will result in suboptimal library quality.