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Atypus karschi Dönitz, 1887 (Araneae: Atypidae): An Asian purse-web spider established in Pennsylvania, USA

Fig 2

Chromosomes of A. karschi, Pennsylvania, USA.

A, B. Male (A) and female karyotypes (B), stained by Giemsa, based on mitotic metaphase. 2n♂ = 41, X0; 2n♀ = 42, XX. Empty arrowhead–centromere of submetacentric pair. C. Male pachytene. Note heterochromatic X chromosome on the periphery of the nucleus and prominent knobs on the bivalents. Inset: Scheme of sex chromosome. Note an association of X chromosome arms. Arrow–sex chromosome. D. Male mitotic metaphase, C-banding. Chromosomes exhibit intercalar and terminal heterochromatin blocks. Inset: Magnified submetacentric chromosome containing a large block of heterochromatin (from another mitotic metaphase). Arrowhead–a large block of heterochromatin, empty arrowhead–centromere. E. Male mitotic metaphase, detection of rDNA cluster (FISH). Note chromosomes of a submetacentric pair containing a terminal rDNA cluster at long arm. Arrowhead–rDNA cluster, empty arrowhead–centromere. Scale bars: 10 μm.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0261695.g002