Discovery of antitumor lectins from rainforest tree root transcriptomes
(A) Merged fluorescent micrographs of A549 cells before (UNT) and after treatment with ML6 for 1 hour and 24 hours. (blue = nucleus, green = lectin). White and yellow arrows highlight punctate regions of ML6 localized to either the cell or nuclear membrane, respectively. Representative micrographs of ML6 localization to the lectin-insensitive (B) MCF7 and (C) NL20 cell lines after 24 hours of incubation shown as controls. Scale bars = 100 μm. Antibody control images can be found in S9 Fig. (D) Normalized cellular fluorescence after 24 hours of incubation with ML6 (shown in relative fluorescence units; RFU).