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Validation of the easyscreen flavivirus dengue alphavirus detection kit based on 3base amplification technology and its application to the 2016/17 Vanuatu dengue outbreak

Fig 2

A diagram showing the 3base conversion process at the nucleotide level.

The bisulphite reaction deaminates cytosine residues in nucleic acids to a uracil sulphonate. After treatment the base is then desulphonated to yield uracil. PCR amplification then converts this to a thymine, which results in essentially a 3base genome (A, G and T).

Fig 2