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Determining the molecular drivers of species-specific interferon-stimulated gene product 15 interactions with nairovirus ovarian tumor domain proteases

Fig 5

Selectively altering CCHFV OTU interactions with human ISG15 and mouse ISG15.

(A) Closeup views of the structural environment encompassing residues 128 and 149 (131/152 in ERVEV) of the OTUs is shown. (B) Overlay of the KUPEV, GANV, and CCHFV OTUs with the targeted sites shown by red dots. (C) Impact of mutations on the activity of CCHFV OTU towards Ub-AMC and human ISG15-AMC. Values shown for the AMC assays represent the mean ± standard deviation of two independent experiments. (D) Impact of mutations on the activity of CCHFV OTU towards human proISG15 and mouse proISG15. Samples from each proISG15 assay timepoint were run on BioRad Mini-PROTEAN® TGX gels and visualized by Coomassie staining.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0226415.g005