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Rapid and highly-specific generation of targeted DNA sequencing libraries enabled by linking capture probes with universal primers

Table 1

LTC 35-gene sequencing performance data for two replicates of sample type and DNA mass.

On-target fraction was defined as the fraction of total bases that aligned to the target regions. Mean target coverage was defined as the mean de-duplicated coverage over all target regions, and uniformity was defined as the fraction of on-target bases that were covered within two fold of the mean target coverage (i.e. between 0.5x and 2x of the mean). Median insert length was measured over all de-duplicated on-target inserts.

Table 1

doi: https://doi.org/10.1371/journal.pone.0208283.t001