Role of extracellular matrix and microenvironment in regulation of tumor growth and LAR-mediated invasion in glioblastoma
(A,B) Time courses of growth/invasion patterns at t = 0, 20, 36, 60 h in the presence of a thick CSPG ring without (A) and with (B) a partial open section. (C,D) Spatial distribution of CSPG in closed and open cases. (E,F) Time courses of levels of [LAR] and [LAR] − [CSGAG] complex at a cell site (cell id = 211, arrowheads in (A4) and (B4)) in closed and open cases. (G,H) Time courses of tumor index (normalized tumor cell populations): total migratory cells (black dashed), migratory cells inside the ring structure (blue solid), and escaped migratory cells (green circle). (I) Number of tumor cells that adhered to the boundary of CSPG ring region at time t = 30, 40, 50, 60 h: cells in Q1,Q2,Q3,Q4 regions in the open case (o-Q), cells in Q1,Q2,Q3,Q4 regions in the closed case (c-Q), cells in the Q1 region in the open case (o-Q1), cells in the Q1 region in the closed case (c-Q1). The Q1,Q2,Q3,Q4 regions were defined in (A1). (J) Number of invasive (escaping) tumor cells in the Q1 section at time t = 30, 40, 50, 60 h in the closed (blue) and open (yellow) cases.