Role of extracellular matrix and microenvironment in regulation of tumor growth and LAR-mediated invasion in glioblastoma
(A-B) Patterns of growth-invasion of a tumor in the presence of low (2.5 × 10−2 μg/mL) and high (250 μg/mL) CSPG at time t = 0 h(A1,B1), 12 h(A2,B2), 24 h(A3,B3), 57 h(A4,B4). (C,D) Patterns of astrocytes at final time (t = 57 h) in response to low and high CSPGs. (E) A high density of astrocytes (blue asterisk ) on the periphery of the tumor in the presence of high CSPG. (F) The number of crossing (invasive) astrocytes in response to low (blue) and high (yellow) CSPGs at time t = 2, 5, 10, 57 h. (G,H,I) Patterns of ramified (pink asterisk) and activated (blue empty circle) microglia at final time (t = 57 h) in response to low and high CSPGs. (J) Activation level of microglia in the presence of low and high CSPGs. (K,L) Populations of growing (blue) and invasive tumor cells in response to low and high CSPGs at time t = 0, 12, 24, 57 h. (M,N) Time courses of concentrations of [LAR] (blue solid) and its complex ([LAR]-[CS-GAG]; red dashed) at a tumor cell site (cellid = 32) in response to low and high CSPGs. Domain size = [60, 140]2 in (A,B,C,D,G,H).