Duplex Proximity Sequencing (Pro-Seq): A method to improve DNA sequencing accuracy without the cost of molecular barcoding redundancy
Pro-Seq RPC was compared to estimates of RPC for duplex and hybrid barcoding using supplementary data . Sequencing efficiency for Pro-Seq was calculated as on-target bases passing all Pro-Seq filters, divided by all (unfiltered) on-target bases (n = 30 runs). Error bars represent maximum and minimum reported values for the relevant data sets. Hybrid barcoding RPC estimates are also comparable to those from .