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Duplex Proximity Sequencing (Pro-Seq): A method to improve DNA sequencing accuracy without the cost of molecular barcoding redundancy

Fig 8

Average reads per consensus (RPC) required to represent a fixed number of input genomes, as a function of cfDNA error rate.

Pro-Seq RPC was compared to estimates of RPC for duplex and hybrid barcoding using supplementary data [18]. Sequencing efficiency for Pro-Seq was calculated as on-target bases passing all Pro-Seq filters, divided by all (unfiltered) on-target bases (n = 30 runs). Error bars represent maximum and minimum reported values for the relevant data sets. Hybrid barcoding RPC estimates are also comparable to those from [24].

Fig 8